Issue 5, 2013
From the journal:

Journal of Materials Chemistry B

High throughput detection and selective enrichment of histidine-tagged enzymes with Ni-doped magnetic mesoporous silica†

Jiho Lee,ab   Soo Youn Lee,a   So Hyun Park,a   Hye Sun Lee,a   Jin Hyung Lee,a   Bong-Yong Jeong,a   Sang-Eon Parkb  and  Jeong Ho Chang*a  

* Corresponding authors

a Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul 153-801, Korea
E-mail: jhchang@kicet.re.kr
Fax: +82 2 3282-7811
Tel: +82 2 3282-249

b Department of Chemistry, Inha University, Incheon 402-751, Korea

Abstract

This work reports a simple and facile method to prepare novel magnetic mesoporous silica (MMS) materials with high magnetic strength for the convenient and high throughput detection of histidine-tagged enzymes with Ni-doped surfaces. These materials are designed by the incorporation of high-abundance and homogeneously dispersed iron nanoparticles within the mesopores by thermal hydrogen reduction after the incorporation of ferrous ions and demonstrated the selective enrichment and high-throughput recognition of His-tagged enzymes with multi-point anchoring by selective conjugation between the His-tag and Ni ions. Selective His-tagged enzyme enrichment efficiency was compared with nickel-based MMS materials, such as Ni2+-MMS and Ni-MMS, and nickel ion doped silica-coated magnetic nanoparticles (Ni2+-MNPs). The efficiency was calculated to be 100 ± 1.93%, 70.94 ± 1.95%, and 37.03 ± 5.93% for Ni2+-MMS, Ni-MMS, and Ni2+-MNPs, respectively. This method enables a high-throughput and advanced systematic approach for the separation and immobilization of proteins which cover a broad spectrum of polyhistidine-tagged proteins.

Graphical abstract: High throughput detection and selective enrichment of histidine-tagged enzymes with Ni-doped magnetic mesoporous silica

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Article information

DOI
https://doi.org/10.1039/C2TB00232A
Article type
Paper
Submitted
24 Aug 2012
Accepted
05 Nov 2012
First published
06 Nov 2012

J. Mater. Chem. B, 2013,1, 610-616

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High throughput detection and selective enrichment of histidine-tagged enzymes with Ni-doped magnetic mesoporous silica

J. Lee, S. Y. Lee, S. H. Park, H. S. Lee, J. H. Lee, B. Jeong, S. Park and J. H. Chang, J. Mater. Chem. B, 2013, 1, 610 DOI: 10.1039/C2TB00232A

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