Issue 32, 2012
From the journal:

Soft Matter

Mechano-chemical control of cell behaviour by elastomer templates coated with biomimetic Layer-by-Layer nanofilms

Fabien Gaudière,a   Ingrid Masson,b   Sandrine Morin-Grognet,a   Olivier Thoumire,a   Jean-Pierre Vannier,c   Hassan Atmani,a   Guy Ladama  and  Béatrice Labat*a  

* Corresponding authors

a Laboratoire de Biophysique et Biomatériaux (La2B, SMS EA 3233), Centre Universitaire d'Evreux, Université de Rouen, 1 rue du 7ème Chasseurs, 27002 Evreux, France
E-mail: beatrice.labat@univ-rouen.fr

b CNRS EAC 4396, Faculté de Médecine, Université Paris-Est Créteil Val de Marne (UPEC), 8 rue du Général Sarrail, 94010 Créteil, France

c Groupe de Recherche sur le Micro-Environnement et le Renouvellement Cellulaire Intégré (MERCI, EA 3829), Faculté de Médecine-Pharmacie, Université de Rouen, 22 boulevard Gambetta, 76183 Rouen, France

Abstract

Within the field of biomaterials, the control of cell–surface interactions is generally addressed through surface chemistry modification. In this respect, Layer-by-Layer (LbL) coatings constitute promising versatile tools. Most cells are mechanosensitive, therefore the stiffness of their microenvironment should also be considered as a crucial parameter when designing polymer-based biomaterials. Here, we report on the combination of mechanics and surface chemistry of LbL-treated polydimethylsiloxane substrates of tunable stiffness to control cell behaviour. Biomimetic LbL films consist of poly-L-lysine (PLL) and chondroitin-4-sulfate (CSA). Stiffness, surface chemistry, wettability and topography of bare and LbL-treated polydimethylsiloxane were analysed. We demonstrated that cells adhered and grew up on all the substrates with significant promotive effects of PLL-terminated films and stiffer substrates. Pre-osteoblasts differentiation was enhanced onto PLL-terminated films irrespective of stiffness and onto CSA-terminated films in a mechanical dependent manner. These results open outlooks within the field of bone tissue engineering, as they demonstrate that osteoconductive surfaces can be obtained by combining uncrosslinked biomimetic LbL films and the intrinsic mechanical properties of the substrates, that is, without any commonly used, potentially cytotoxic chemical crosslinking.

Graphical abstract: Mechano-chemical control of cell behaviour by elastomer templates coated with biomimetic Layer-by-Layer nanofilms

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Article information

DOI
https://doi.org/10.1039/C2SM25614B
Article type
Paper
Submitted
16 Mar 2012
Accepted
01 Jun 2012
First published
03 Jul 2012

Soft Matter, 2012,8, 8327-8337

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Mechano-chemical control of cell behaviour by elastomer templates coated with biomimetic Layer-by-Layer nanofilms

F. Gaudière, I. Masson, S. Morin-Grognet, O. Thoumire, J. Vannier, H. Atmani, G. Ladam and B. Labat, Soft Matter, 2012, 8, 8327 DOI: 10.1039/C2SM25614B

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