Issue 11, 2012
From the journal:

Analyst

Label-free detection of proteins from dried-suspended droplets using surface enhanced Raman scattering†

Sercan Keskina  and  Mustafa Çulha*a  

* Corresponding authors

a Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Atasehir, Istanbul 34755, Turkey
E-mail: mculha@yeditepe.edu.tr
Fax: +90 (216)5780829
Tel: +90 (216)5781587

Abstract

A simple sample preparation method to obtain rich and reproducible surface-enhanced Raman scattering (SERS) spectra from proteins regardless of their surface properties and dimensions for label-free detection and identification is reported. The method uses colloidal silver nanoparticles (AgNPs) as substrates and is based on suspending the droplet of a mixture containing AgNPs and proteins from a hydrophobic surface. Drying the droplet at this suspended configuration allows the accumulation and packing of AgNPs and protein molecules in the middle of the droplet area rather than getting jammed at the edges of drying droplets as the solvent evaporates. A detection limit of down to 0.05 μg mL−1 for some of the model proteins used in this study is obtained with this simple approach. The advantage of this method is its simplicity and improved sensitivity over other approaches reported in the literature.

Graphical abstract: Label-free detection of proteins from dried-suspended droplets using surface enhanced Raman scattering

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Article information

DOI
https://doi.org/10.1039/C2AN16296B
Article type
Paper
Submitted
23 Dec 2011
Accepted
29 Mar 2012
First published
30 Mar 2012

Analyst, 2012,137, 2651-2657

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Label-free detection of proteins from dried-suspended droplets using surface enhanced Raman scattering

S. Keskin and M. Çulha, Analyst, 2012, 137, 2651 DOI: 10.1039/C2AN16296B

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