Issue 11, 2011
From the journal:

Organic & Biomolecular Chemistry

Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation†

Kaori Sugizaki,a   Shuji Ikeda,a   Hiroyuki Yanagisawaa  and  Akimitsu Okamoto*ab  

* Corresponding authors

a Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan
E-mail: aki-okamoto@riken.jp
Fax: +81 48 467 9205
Tel: +81 48 467 9238

b PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

DNA strands containing a 5-hydroxymethylcytosine (hmC), which have recently been found in neuron cells and embryonic stem cells, were synthesized through a facile synthetic technique. The hmC-containing strands were efficiently oxidized at hmC using an osmium oxidation assay. The hmC was oxidized as easily as 5-methylcytosine, which can be distinguished from unmethylated cytosine.

Graphical abstract: Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

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Article information

DOI
https://doi.org/10.1039/C1OB05247K
Article type
Paper
Submitted
16 Feb 2011
Accepted
28 Mar 2011
First published
28 Mar 2011

Org. Biomol. Chem., 2011,9, 4176-4181

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Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

K. Sugizaki, S. Ikeda, H. Yanagisawa and A. Okamoto, Org. Biomol. Chem., 2011, 9, 4176 DOI: 10.1039/C1OB05247K

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