Issue 24, 2011
From the journal:

Dalton Transactions

Label-free emission assay of mercuric ions using DNA duplexes of poly(dT)†

Byul Nim Oh,a   Sangji Park,a   Jun Ren,a   Yoon Jung Jang,b   Seog K. Kimb  and  Jinheung Kim*a  

* Corresponding authors

a Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea
E-mail: jinheung@ewha.ac.kr
Fax: +82-2-3277-3419
Tel: +82-2-3277-4453

b Department of Chemistry, Youngnam University, Deagu, Korea

Abstract

In this study, an assay to quantify the presence of mercuric ions and methyl mercury by double-stranded DNA containing a poly(dT) sequence was developed using a light switch compound, Ru(phen)2(dppz)2+ (1), which is known to intercalate into double-stranded DNA. Upon treatment with mercuric ions, the metal-to-ligand charge transfer (MLCT) emission derived from the intercalation of 1 was reduced due to the formation of DNA duplexes containing T–Hg2+–T base pairs by the dehybridization of poly(dT)–poly(dA) duplexes at room temperature. As the concentration of Hg2+ was increased, the emission of 1 gradually decreased. This label-free method had a detection limit of 5 nM. Other metal ions, such as K+, Ag+, Ca2+, Mg2+, Zn2+, Mn2+, Co2+, Ni2+, Cu2+, Cd2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the dehybridization rate of dsDNA and the rate decreased in the order of T10C·A11 ∼ T10A·A11 > T10G·A11 > T11·A11.

Graphical abstract: Label-free emission assay of mercuric ions using DNA duplexes of poly(dT)

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1DT10083A
Article type
Paper
Submitted
17 Jan 2011
Accepted
14 Mar 2011
First published
12 May 2011

Dalton Trans., 2011,40, 6494-6499

Permissions

Request permissions

Label-free emission assay of mercuric ions using DNA duplexes of poly(dT)

B. N. Oh, S. Park, J. Ren, Y. J. Jang, S. K. Kim and J. Kim, Dalton Trans., 2011, 40, 6494 DOI: 10.1039/C1DT10083A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements