Issue 19, 2011
From the journal:

Analyst

Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

Sabine Zakel,*a   Olaf Rienitz,a   Bernd Güttlera  and  Rainer Stoscha  

* Corresponding authors

a Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, Braunschweig, Germany
E-mail: Sabine.Zakel@ptb.de

Abstract

Double isotope dilution surface-enhanced Raman scattering (double IDSERS) is qualified as a method for accurate and precise determination of biomarkers in human blood serum. Providing a full evaluation of the measurement uncertainty as well as traceability to a reference material sets the procedure in line with the requirements of a primary ratio method. Data evaluation is based on a partial least squares (PLS) model, whose prediction ability is validated from quantifying the uric acid concentration in both an artificial reference solution and a real human blood serum sample. With the proposed approach, the uric acid serum concentration can be determined with an uncertainty of 1.6% at a confidence level of 95%.

Graphical abstract: Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

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Article information

DOI
https://doi.org/10.1039/C1AN15207F
Article type
Paper
Submitted
11 Mar 2011
Accepted
28 Jun 2011
First published
05 Aug 2011

Analyst, 2011,136, 3956-3961

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Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

S. Zakel, O. Rienitz, B. Güttler and R. Stosch, Analyst, 2011, 136, 3956 DOI: 10.1039/C1AN15207F

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