Issue 12, 2010

Single-step bifunctional coating for selectively conjugable nanoparticles

Abstract

A single-step method to coat and bifunctionalize water-reduced gold nanoparticles (NPs) with two distinct reactive groups is reported. The coating is based on a peptide that bonds to the NPs surface by its N-cysteine amino acid, terminates with a C-terminal lysine, and stabilizes the colloids, thanks to the surface organization provided by the rest of the non-polar chain. The process yields stable, non-cytotoxic NPs presenting reactive amine and carboxylic groups on the surface; these allow rapid, selective and modular conjugation of virtually any chosen biomolecule or fluorophore. Functionalized and conjugated nanostructures are analyzed by electrophoresis, SEM, SERS; their biocompatibility and delivery capability are tested by cellular-uptake experiments.

Graphical abstract: Single-step bifunctional coating for selectively conjugable nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
28 May 2010
Accepted
02 Sep 2010
First published
11 Oct 2010

Nanoscale, 2010,2, 2783-2789

Single-step bifunctional coating for selectively conjugable nanoparticles

V. Voliani, S. Luin, F. Ricci and F. Beltram, Nanoscale, 2010, 2, 2783 DOI: 10.1039/C0NR00350F

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