Issue 23, 2010
From the journal:

Lab on a Chip

In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device

Keun Pil Kim,†a   Yun-Gon Kim,†b   Chang-Hyung Choi,†c   Hye-Eun Kim,c   Sang-Ho Lee,d   Woo-Suk Chang*e  and  Chang-Soo Lee*c  

* Corresponding authors

a Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA

b Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA

c Department of Chemical Engineering, Chungnam National University, Yuseong-gu, Daejeon, South Korea
E-mail: rhadum@cnu.ac.kr

d Fusion Technology R&D Division, Korea Institute of Industrial Technology, Ansan, South Korea

e Department of Biology, University of Texas-Arlington, Arlington, Texas, USA
E-mail: wschang@uta.edu

Abstract

Antibiotic resistance of biofilms is a growing public health concern due to overuse and improper use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate bacterial biofilms is crucial. Here we present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the concentration required to eradicate biofilm-grown Pseudomonas aeruginosa is higher than the minimal inhibitory concentration (MIC) that has been widely used to determine the lowest concentration of antibiotics against planktonically grown bacteria.

Graphical abstract: In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device

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Article information

DOI
https://doi.org/10.1039/C0LC00154F
Article type
Technical Note
Submitted
30 Jun 2010
Accepted
17 Aug 2010
First published
11 Oct 2010

Lab Chip, 2010,10, 3296-3299

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In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device

K. P. Kim, Y. Kim, C. Choi, H. Kim, S. Lee, W. Chang and C. Lee, Lab Chip, 2010, 10, 3296 DOI: 10.1039/C0LC00154F

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