Issue 48, 2010
From the journal:

Chemical Communications

Fluorogenic assay and live cell imaging of HIV-1protease activity using acid-stable quantum dot–peptide complex†

Youngseon Choi,a   Junghan Lee,a   Keumhyun Kim,a   Heeyeon Kim,a   Peter Sommerb  and  Rita Song*a  

* Corresponding authors

a Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 696 Sampyeong-dong, Bundang-gu, Seongnam-Si, Gyeonggi-Do, South Korea
E-mail: rsong@ip-korea.org
Fax: +82-31-8018-8014
Tel: +82-31-8018-8230

b Cell Biology of Retroviruses Laboratory, Institut Pasteur Korea (IP-K), South Korea

Abstract

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

Graphical abstract: Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot–peptide complex

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Article information

DOI
https://doi.org/10.1039/C0CC02702B
Article type
Communication
Submitted
21 Jul 2010
Accepted
07 Oct 2010
First published
04 Nov 2010

Chem. Commun., 2010,46, 9146-9148

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Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot–peptide complex

Y. Choi, J. Lee, K. Kim, H. Kim, P. Sommer and R. Song, Chem. Commun., 2010, 46, 9146 DOI: 10.1039/C0CC02702B

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