Issue 5, 2010
From the journal:

Polymer Chemistry

Specific interaction of DNA-functionalized polymer colloids

Rungsima Chollakup,*a   Wirasak Smitthiponga  and  Arkadiusz Chworos*b  

* Corresponding authors

a Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Phaholyotin Rd, Chatuchak, Bangkok, Thailand
E-mail: aaprmc@ku.ac.th
Fax: (+662) 9405634
Tel: (+662) 9428604

b Department of Physics, University of California, Santa Barbara, CA, USA
E-mail: achworos@physics.ucsb.edu
Tel: (+805) 8933672

Abstract

As a chemically based assembly system, DNA is a key player for use in the reversible and specific interactions of an advanced material. In this approach, we work with a one-component system where a number of DNA single strands are grafted on the surface of micro-sized particles. The interactions between the DNA-functionalized particles are controlled by the addition of DNA linker in solution, which has complementary sequences with the DNA strands grafted on the particles. The hydrogen bonding based on Watson-Crick pairing is the driving force to form double stranded DNA. Several techniques have been used to characterize this system; for example, optical microscopy, AFM, DLS and flow cytometry. The two DNA strands reversibly separate (denature) above a characteristic melting temperature of DNA's base pair sequences. The melting temperature of DNA in the study is in good agreement with that of calculation based on the thermodynamic point of view.

Graphical abstract: Specific interaction of DNA-functionalized polymer colloids

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Article information

DOI
https://doi.org/10.1039/B9PY00290A
Article type
Paper
Submitted
10 Oct 2009
Accepted
12 Jan 2010
First published
05 Feb 2010

Polym. Chem., 2010,1, 658-662

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Specific interaction of DNA-functionalized polymer colloids

R. Chollakup, W. Smitthipong and A. Chworos, Polym. Chem., 2010, 1, 658 DOI: 10.1039/B9PY00290A

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