Issue 1, 2010
From the journal:

Soft Matter

Differential adhesion of fibroblast and neuroblastoma cells on size- and geometry-controlled nanofibrils of the extracellular matrix†

Peter Kaiserab  and  Joachim P. Spatz*ab  

* Corresponding authors

a Department of New Materials and Biosystems, Max-Planck-Institute for Metals Research, Heisenbergstrasse 3, Stuttgart, Germany
E-mail: spatz@mf.mpg.de

b Department of Biophysical Chemistry, University of Heidelberg, Germany, INF 253, Heidelberg, Germany
E-mail: Joachim.Spatz@urz.uni-heidelberg.de

Abstract

We present a universal method to produce highly regular arrays of nanofibrils constituted by the extracellular matrix (ECM) proteins fibronectin (FN), collagen I (COL I) and EHS laminin (EHS LN). Adjusting the de-wetting parameters of a protein solution on a hydrophobic silicon micropillar structure, we control the mean nanofibril diameter between 20 nm and 160 nm. Array geometry predetermines nanofibril diameter, while protein concentration and de-wetting speed have to be adapted to yield high regularity. ECM nanofibrils span the space between adjacent pillars and immunofluorescence labelling of LN and FN nanofibrils allows us to discriminate both species in mixed networks. The biological function of ECM nanofibrils was verified in cell adhesion experiments after transfer of the nanofibrils onto non-adhesive polyethylene glycol (PEG) hydrogels. Co-cultures of fibroblasts and neuroblastoma cells on vertically arranged nanofibrils of FN and LN showed differential adhesion of the distinct cell types.

Graphical abstract: Differential adhesion of fibroblast and neuroblastoma cells on size- and geometry-controlled nanofibrils of the extracellular matrix

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B911461K
Article type
Paper
Submitted
11 Jun 2009
Accepted
15 Sep 2009
First published
12 Oct 2009

Soft Matter, 2010,6, 113-119

Permissions

Request permissions

Differential adhesion of fibroblast and neuroblastoma cells on size- and geometry-controlled nanofibrils of the extracellular matrix

P. Kaiser and J. P. Spatz, Soft Matter, 2010, 6, 113 DOI: 10.1039/B911461K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements