Issue 8, 2008
From the journal:

Green Chemistry

Accumulation of ionic liquids in Escherichia colicells

Robert J. Cornmell,a   Catherine L. Winder,b   Gordon J. T. Tiddy,a   Royston Goodacreb  and  Gill Stephens*a  

* Corresponding authors

a School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK
E-mail: gill.stephens@manchester.ac.uk
Fax: +44 161 3068918
Tel: +44 161 3064377

b School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK

Abstract

Ionic liquids accumulate within Escherichia colicells and can be detected by Fourier transform infrared (FT-IR) spectroscopy. Harvested cells were incubated with the biocompatible, water-immiscible ionic liquids, trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([P6,6,6,14][NTf2]) and methyltrioctylammonium bis(trifluoromethylsulfonyl)imide ([N1,8,8,8][NTf2]), and with the toxic chlorides, [P6,6,6,14][Cl] and [N1,8,8,8][Cl]. The cells were harvested, washed and dried, and their FT-IR spectra were recorded. The ionic liquid spectra could be detected against the background spectra of the cells, demonstrating that they were accumulating within the cells. The toxic ionic liquids accumulated more rapidly than the biocompatible ionic liquids. Principal components analysis followed by discriminant function analysis showed that, compared to control cells, the toxic ionic liquids produced much bigger changes in the FT-IR fingerprint of the cellular chemicals than the biocompatible ionic liquids. Subcellular fractionation, followed by FT-IR analysis, demonstrated that [P6,6,6,14][NTf2] accumulated specifically in the membrane fraction of the cells and not the cytoplasm.

Graphical abstract: Accumulation of ionic liquids in Escherichia colicells

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Article information

DOI
https://doi.org/10.1039/B807214K
Article type
Paper
Submitted
28 Apr 2008
Accepted
11 Jun 2008
First published
04 Jul 2008

Green Chem., 2008,10, 836-841

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Accumulation of ionic liquids in Escherichia colicells

R. J. Cornmell, C. L. Winder, G. J. T. Tiddy, R. Goodacre and G. Stephens, Green Chem., 2008, 10, 836 DOI: 10.1039/B807214K

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