Issue 1, 2008
From the journal:

Soft Matter

Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry†

Andrea R. Statz,a   Annelise E. Barron‡bc  and  Phillip B. Messersmith*acd  

* Corresponding authors

a Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL, USA
E-mail: philm@northwestern.edu
Fax: +1 847 491 4928
Tel: +1 847 467 5273

b Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL, USA

c Institute for BioNanotechnology in Medicine, Northwestern University, 2145 Sheridan Rd, Evanston, IL, USA

d Department of Materials Science and Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL, USA

Abstract

Peptidomimetic polymers consisting of poly-N-substituted glycine oligomers (polypeptoids) conjugated to biomimetic adhesive polypeptides were investigated as antifouling surface coatings. The polymers were immobilized onto TiO2 surfaces via an anchoring peptide consisting of alternating residues of 3,4-dihydroxyphenylalanine (DOPA) and lysine. Three polypeptoid side-chain compositions were investigated for antifouling performance and stability toward enzymatic degradation. Ellipsometry and XPS analysis confirmed that purified polymers adsorbed strongly to TiO2 surfaces, and the immobilized polymers were resistant to enzymatic degradation as demonstrated by mass spectrometry. All polypeptoid-modified surfaces exhibited significant reductions in adsorption of lysozyme, fibrinogen and serum proteins, and were resistant to 3T3 fibroblast cell attachment for up to seven days. Long-term in vitro cell attachment studies conducted for six weeks revealed the importance of polypeptoid side-chain composition, with a methoxyethyl side chain providing superior long-term fouling resistance compared to hydroxyethyl and hydroxypropyl side chains. Finally, attachment of both gram-positive and gram-negative bacteria for up to four days under continuous-flow conditions was significantly reduced on the polypeptoid-modified surfaces compared to unmodified TiO2 surfaces. The results reveal the influence of polypeptoid side-chain chemistry on short-term and long-term protein, cell and bacterial fouling resistance.

Graphical abstract: Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry

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Article information

DOI
https://doi.org/10.1039/B711944E
Article type
Paper
Submitted
03 Aug 2007
Accepted
19 Sep 2007
First published
12 Oct 2007

Soft Matter, 2008,4, 131-139

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Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry

A. R. Statz, A. E. Barron and P. B. Messersmith, Soft Matter, 2008, 4, 131 DOI: 10.1039/B711944E

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