Issue 30, 2006
From the journal:

Chemical Communications

Selection of DNA ligands for protein kinase C-δ†

Prabodhika Mallikaratchy,a   Robert V. Stahelin,b   Zehui Cao,a   Wonhwa Chob  and  Weihong Tan*a  

* Corresponding authors

a Center for Research at the Bio/nano Interface, Department of Chemistry, Shands Cancer Centre, University of Florida, Gainesville, USA
E-mail: tan@chem.ufl.edu
Tel: 352-846-2410

b Department of Chemistry, University of Illinois at Chicago, Chicago, IL, USA

Abstract

Protein kinase Cs are a family of serine and threonine kinases that mediate a wide variety of cellular signalling processes such as cell growth, differentiation, apoptosis and tumor development. We have selected high-affinity DNA aptamers for PKCδ by capillary electrophoresis based SELEX (systematic evolution of ligands by exponential enrichment, CE-SELEX). We have demonstrated that fluorescently tagged PB9 aptamer can specifically recognize PKCδ under in vitro conditions. The Kd of the aptamer-protein binding is 122 nM. These aptamers will enable us to apply fluorescently labelled probes to study the spatiotemporal dynamics and activation of individual endogenous PKC isoforms during various cell signalling processes.

Graphical abstract: Selection of DNA ligands for protein kinase C-δ

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Article information

DOI
https://doi.org/10.1039/B604778E
Article type
Communication
Submitted
03 Apr 2006
Accepted
24 May 2006
First published
23 Jun 2006

Chem. Commun., 2006, 3229-3231

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Selection of DNA ligands for protein kinase C-δ

P. Mallikaratchy, R. V. Stahelin, Z. Cao, W. Cho and W. Tan, Chem. Commun., 2006, 3229 DOI: 10.1039/B604778E

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