Issue 12, 2002
From the journal:

Analyst

Development of a sequential enzymolysis approach for the evaluation of the bioaccessibility of Cd and Pb from cocoa

S. Mounicou,a   J. Szpunar,a   D. Andrey,b   C. Blakeb  and  R. Lobinski*a  

* Corresponding authors

a Equipe de Chimie Analytique Bio-inorganique, CNRS UMR 5034, Hélioparc, 2, av. Pr. Angot, Pau, France

b NESTEC, Vers chez les Blanc, Lausanne 26, Switzerland

Abstract

An in vitro model simulating enzymatic activity in the gastrointestinal tract was developed for the assessment of the potential bioaccessibility of Cd and Pb in cocoa powder and liquor. The model was based on the sequential extraction with simulated gastric and intestinal juices; the residue after the latter extraction was further investigated by using, in parallel, solutions of phytase and cellulase. The solubility of Cd and Pb in the corresponding enzymatic extracts was measured by ICP MS. The bioaccessibility of Cd in cocoa varied from 10 to 50% in gastrointestinal conditions. An additional 20 or 30% of Cd could be recovered by phytase and cellulase, respectively. The bioaccessibility of Pb in gastrointestinal conditions did not exceed 5–10%. Only a few percent more of this metal could be recovered by extraction with phytase and cellulase.

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Article information

DOI
https://doi.org/10.1039/B207427N
Article type
Paper
Submitted
29 Jul 2002
Accepted
07 Nov 2002
First published
18 Nov 2002

Analyst, 2002,127, 1638-1641

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Development of a sequential enzymolysis approach for the evaluation of the bioaccessibility of Cd and Pb from cocoa

S. Mounicou, J. Szpunar, D. Andrey, C. Blake and R. Lobinski, Analyst, 2002, 127, 1638 DOI: 10.1039/B207427N

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