Issue 2, 2002
From the journal:

Analyst

Mixed enzyme (laccase/tyrosinase)-based remote electrochemical biosensor for monitoring phenolic compounds

Renato S. Freire,a   Sompong Thongngamdee,b   Nelson Durán,ac   Joseph Wang*b  and  Lauro T. Kubota*a  

* Corresponding authors

a Instituto de Química, Universidade Estadual de Campinas –UNICAMP, CP 6154, CEP 13083-970 Campinas, SP, Brazil
E-mail: kubota@iqm.unicamp.br
Fax: +55 19 7883023
Tel: +55 19 7883127

b Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, USA
E-mail: joewang@nmsu.edu
Fax: +1 505 646 6033
Tel: +1 505 646 2140

c Núcleo de Ciências Ambientais, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil

Abstract

An electrochemical biosensor for remote continuous monitoring of phenolic compounds in environmental analysis is described. The probe relies on rapid and sensitive amperometric detection at a submersible biosensor assembly, connected to a 50 ft long shielded cable. The enzymes laccase and tyrosinase were used as individual sensors and also as a bienzymatic sensor; these enzymes were immobilized chemically on the carbon fiber transducer. The analysis was based on the amperometric detection of the enzymatic products at a potential of −0.10 V vs. Ag/AgCl. Operational conditions were optimized to meet the requirements of remote operations. Tests with untreated river water spiked with phenolic compounds gave results similar to those obtained with synthetic buffer solutions. The remote laccase biosensor allowed the convenient quantification of guaiacol and chloroguaiacol at levels down to 22 and 9 nmol L−1, respectively. The co-immobilization of laccase and tyrosinase allowed the efficient detection of a larger group of phenolic compounds.

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Article information

DOI
https://doi.org/10.1039/B110011D
Article type
Paper
Submitted
02 Nov 2001
Accepted
03 Dec 2001
First published
11 Jan 2002

Analyst, 2002,127, 258-261

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Mixed enzyme (laccase/tyrosinase)-based remote electrochemical biosensor for monitoring phenolic compounds

R. S. Freire, S. Thongngamdee, N. Durán, J. Wang and L. T. Kubota, Analyst, 2002, 127, 258 DOI: 10.1039/B110011D

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