Skip to main content
SpringerLink
Log in

Glucose oxidase catalyses the reduction of O2 to H2O2 in the presence of irradiated TiO2 and isopropyl alcohol

  • Paper
  • Published:
Photochemical & Photobiological Sciences Aims and scope Submit manuscript

Abstract

Catalytic amounts of glucose oxidase from Aspergillus niger (GO) are active in the reduction of O2 to H2O2 in the presence of irradiated suspensions of TiO2 and isopropyl alcohol as electron donor. An explanation of this behaviour is given on the basis of the ability of the enzyme to capture electrons from the photoexcited TiO2 instead of its natural substrate, glucose. This process has a marked positive effect on both the oxidation of isopropyl alcohol to acetone and the formation of radical intermediates, which have been detected, for the first time, by EPR—spin trapping investigation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. Gerischer, A. Heller, J. Phys. Chem., 1991, 95, 5261.

    Article  CAS  Google Scholar 

  2. C.-M. Wang, A. Heller, H. Gerischer, J. Am. Chem. Soc., 1992, 114, 5230.

    Article  CAS  Google Scholar 

  3. L. Sun, J. R. Bolton, J. Phys. Chem., 1996, 100, 4127.

    Article  CAS  Google Scholar 

  4. J. M. Kesselman, G. A. Shreve, M. R. Hoffmann, N. S. Lewis, J. Phys. Chem., 1994, 98, 13385.

    Article  CAS  Google Scholar 

  5. L. Cermenati, P. Pichat, C. Guillard, A. Albini, J. Phys. Chem., 1997, 101, 2650.

    Article  CAS  Google Scholar 

  6. J. Schwitzgebel, J. G. Ekerdt, H. Gerischer, A. Heller, J. Phys. Chem., 1995, 99, 5633.

    Article  CAS  Google Scholar 

  7. P. Calza, C. Minero, E. Pelizzetti, J. Chem. Soc., Faraday Trans., 1997, 93, 3765.

    Article  CAS  Google Scholar 

  8. H. J. Hecht, H. M. Kalisz, J. Hendle, R. D. Schmid, D. Schomburg, J. Mol. Biol., 1993, 229, 153.

    Article  CAS  Google Scholar 

  9. S. Cosnier, A. Senillou, M. Grätzel, P. Comte, N. Vlachopoulos, N. J. Renault, C. Martelet, J. Electroanal. Chem., 1999, 469, 176.

    Article  CAS  Google Scholar 

  10. G. M. Mura, M. L. Ganadu, G. Lubinu, V. Maida, Ann. N. Y. Acad. Sci., 1999, 879, 267.

    Article  CAS  Google Scholar 

  11. G. M. Mura, M. L. Ganadu, P. Lombardi, G. Lubinu, M. Branca, V. Maida, J. Photochem. Photobiol. A: Chem., 2002, 148, 199.

    Article  CAS  Google Scholar 

  12. A. Molinari, R. Amadelli, L. Antolini, A. Maldotti, P. Battioni, D. Mansuy, J. Mol. Catal. A: Chem., 2000, 158, 521.

    Article  CAS  Google Scholar 

  13. A. Maldotti, A. Molinari, R. Amadelli, Chem. Rev., 2002, 102, 3811.

    Article  CAS  Google Scholar 

  14. M. K. Nazeeruddin, A. Kay, I. Rodicio, R. Humphry-Baker, E. Muller, P. Liska, N. Vlachopoulos, M. Gratzel, J. Am. Chem. Soc., 1993, 115, 6382.

    Article  CAS  Google Scholar 

  15. S. P. Wolff, Methods Enzymol., 1994, 233, 182.

    Article  CAS  Google Scholar 

  16. K. K. Makinen, J. Tenovuo, Anal. Biochem., 1982, 126, 100.

    Article  CAS  Google Scholar 

  17. I. Okura, Coord. Chem. Rev., 1985, 68, 53.

    Article  CAS  Google Scholar 

  18. J. Kyte, Mechanism in Protein Chemistry, Garland Publishing Inc., New York & London, 1995, ch. 2.

  19. R. Konaka, E. Kasahara, W. C. Dunlap, Y. Yamamoto, K. C. Chien, M. Inoue, Free Radicals Biol. Med., 1999, 27, 294.

    Article  CAS  Google Scholar 

  20. V. Agugliaro, E. Davi, L. Palmisano, M. Schiavello, A. Sclafani, Appl. Catal., 1990, 65, 101.

    Article  Google Scholar 

  21. I. Ilisz, K. Föglein, A. Dombi, J. Mol. Catal. A: Chem., 1998, 135, 55.

    Article  CAS  Google Scholar 

  22. M. A. Fox, H. Ogawa, P. Pichat, J. Org. Chem., 1989, 54, 3847.

    Article  CAS  Google Scholar 

  23. G. R. Buettner, Free Radicals Biol. Med., 1987, 3, 259.

    Article  CAS  Google Scholar 

  24. R. Amadelli, A. Maldotti, C. Bartocci, V. Carassiti, J. Phys. Chem., 1989, 93, 6448.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Chimica, Università di Sassari, Via Vienna 2, 07100, Sassari, Italy

    Maria L. Ganadu & Giovanni M. Mura

  2. Dipartimento di Chimica, Università di Ferrara, ISOF C.N.R Sezione di Ferrara, Via L. Borsari 46, 44100, Ferrara, Italy

    Leonardo Andreotti, Irene Vitali, Andrea Maldotti & Alessandra Molinari

Authors
  1. Maria L. Ganadu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leonardo Andreotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Irene Vitali
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrea Maldotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alessandra Molinari
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Giovanni M. Mura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria L. Ganadu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ganadu, M.L., Andreotti, L., Vitali, I. et al. Glucose oxidase catalyses the reduction of O2 to H2O2 in the presence of irradiated TiO2 and isopropyl alcohol. Photochem Photobiol Sci 1, 951–954 (2002). https://doi.org/10.1039/b109953c

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1039/b109953c

Search

Navigation