Issue 12, 1994
From the journal:

Analyst

Determination of xanthines by high-performance liquid chromatography and thin-layer chromatography in horse urine after ingestion of guaraná powder

Myriam C. Salvadori,   Evelyn M. Rieser,   Luciane M. Ribeiro Neto  and  Elizabeth S. Nascimento  

Abstract

The seeds of Guaraná are rich in xanthines and are used for the preparation of guaraná powder which is very commonly given to horses as a ‘tonic’ in Brazil. In this paper, the xanthine content of guaraná powder was determined, in addition to its clearance time in horses. Thin-layer chromatography was used as a screening procedure and high-performance liquid chromatography was performed to quantify the drugs in both the powder and urine samples. The guaraná powder was found to contain 2.16, 1.10 and 36.78 mg g–1 of theobromine (TB), theophylline (TP) and caffeine (CF), respectively, and in urine it was possible to detect TB and TP up to 13 d and CF up to 9 d after the administration of guaraná powder.

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Article information

DOI
https://doi.org/10.1039/AN9941902701
Article type
Paper

Analyst, 1994,119, 2701-2703

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Determination of xanthines by high-performance liquid chromatography and thin-layer chromatography in horse urine after ingestion of guaraná powder

M. C. Salvadori, E. M. Rieser, L. M. R. Neto and E. S. Nascimento, Analyst, 1994, 119, 2701 DOI: 10.1039/AN9941902701

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