Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)

Hugues Bonnet; Maéva Morel; Alexandre Devaux; Joseph Boissieras; Anton Granzhan; Benjamin Elias; Thomas Lavergne; Jérôme Dejeu; Eric Defrancq

doi:10.1039/D2CC00836J

Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)†

Hugues Bonnet,^a Maéva Morel,^a Alexandre Devaux,^a Joseph Boissieras,^b Anton Granzhan,

^b Benjamin Elias,

^c Thomas Lavergne,

*^a Jérôme Dejeu

*^ad and Eric Defrancq

*^a

Author affiliations

* Corresponding authors

^a Université Grenoble Alpes, Département de Chimie Moléculaire, UMR CNRS 5250, CS 40700-38058 Grenoble, France
E-mail: eric.defrancq@univ-grenoble-alpes.fr, jerome.dejeu@univ-grenoble-alpes.fr, thomas.lavergne@univ-grenoble-alpes.fr

^b Institut Curie, CNRS UMR 9187, INSERM U1196, Université Paris Saclay, 91405 Orsay, France

^c Institut de la Matière Condensée et des Nanosciences (IMCN), Molecular Chemistry, Materials and Catalysis (MOST), Université catholique de Louvain (UCLouvain), Place Louis Pasteur 1, 1348 Louvain-la-Neuve, Belgium

^d FEMTO-ST Institute, CNRS UMR-6174, Université de Bourgogne Franche-Comté, F-25000 Besançon, France

Abstract

Biolayer interferometry (BLI) and circular dichroism (CD) spectroscopy were used to investigate the interaction between previously reported i-motif DNA (i-DNA) ligands and folded or unfolded i-DNA in acidic (pH 5.5) and near-neutral (pH 6.5) conditions. We observed that although several ligands, in particular macrocyclic bis-acridine (BisA) and pyridostatin (PDS), showed good affinities for the telomeric i-motif forming sequence, none of the ligands displayed selective interactions with the i-DNA structure nor was able to promote its formation.

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DOI: https://doi.org/10.1039/D2CC00836J
Article type: Communication
Submitted: 10 Feb 2022
Accepted: 26 Mar 2022
First published: 30 Mar 2022
This article is Open Access

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Chem. Commun., 2022,58, 5116-5119

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Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)

H. Bonnet, M. Morel, A. Devaux, J. Boissieras, A. Granzhan, B. Elias, T. Lavergne, J. Dejeu and E. Defrancq, Chem. Commun., 2022, 58, 5116 DOI: 10.1039/D2CC00836J

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Chemical Communications

Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)†

Abstract

Supplementary files

Article information

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Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)

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