Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent

Danica Z. Zmejkoski; Zoran M. Marković; Nemanja M. Zdravković; Dijana D. Trišić; Milica D. Budimir; Sanja B. Kuzman; Natalia O. Kozyrovska; Iryna V. Orlovska; Nikol Bugárová; Đorđe Ž. Petrović; Mária Kováčová; Angela Kleinová; Zdeno Špitalský; Vladimir B. Pavlović; Biljana M. Todorović Marković

doi:10.1039/D0RA10782D

Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent

Danica Z. Zmejkoski,

*^a Zoran M. Marković,^a Nemanja M. Zdravković,^b Dijana D. Trišić,

^c Milica D. Budimir,^a Sanja B. Kuzman,^a Natalia O. Kozyrovska,

^d Iryna V. Orlovska,^d Nikol Bugárová,

^e Đorđe Ž. Petrović,^a Mária Kováčová,^e Angela Kleinová,

^e Zdeno Špitalský,^e Vladimir B. Pavlović^f and Biljana M. Todorović Marković*^a

Author affiliations

* Corresponding authors

^a Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia
E-mail: danica@vinca.rs, biljatod@vinca.rs, biljatod@vin.bg.ac.rs
Tel: +381 113408582

^b Scientific Veterinary Institute of Serbia, Janisa Janulisa 14, 11107 Belgrade, Serbia

^c Faculty of Dental Medicine, University of Belgrade, Dr. Subotića 8, 11000 Belgrade, Serbia

^d Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150, Zabolotnogo Str., Kyiv, Ukraine

^e Polymer Institute, Slovak Academy of Sciences, Dúbravska cestá 9, 84541 Bratislava, Slovakia

^f Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia

Abstract

Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materials in its three dimensional network structure. In this work a low molecular weight chitosan (Chi) polymer is incorporated in BC with different concentrations. Different characterization techniques are used to investigate structural and optical properties of these composites. Radical scavenging activity test shows moderate antioxidant activity of these biocompatible composites whereas in vitro release test shows that 13.3% of chitosan is released after 72 h. Antibacterial testing of BC–Chi composites conducted on Gram-positive and Gram-negative bacteria causing UTIs and CAUTIs (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) and encrustation (Proteus mirabilis) show bactericidal effect. The morphology analysis of bacteria after the application of BC–Chi shows that they are flattened with a rough surface, with a tendency to agglomerate and with decreased length and width. All obtained results show that BC–Chi composites might be considered as potential biomedical agents in treatment of UTIs and CAUTIs and as a urinary catheter coating in encrustation prevention.

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Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent

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Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent

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