The fabrication of a cationic polyacrylamide (CPAM) with high efficiency and economy has been highly desired in the field of high-turbidity water treatment. This study introduced an ultrasound (US)-initiated template polymerization (UTP) method to develop a novel cationic templated polyacrylamide (TPAA) with a microblock structure. TPAA was prepared using acrylamide (AM) and sodium (3-acrylamidopropyl)trimethylammonium chloride (ATAC) as the monomers and sodium polyacrylate (NaPAA) as the template. Factors that affected polymerization such as the ultrasound power, ultrasound time, initiator concentration, pH, and m_AM : m_ATAC and n_NaPAA : n_ATAC values were investigated. The properties of the polymers were characterized by Fourier transform infrared spectroscopy (FTIR), ¹H nuclear magnetic resonance spectroscopy (¹H NMR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The results indicated the successful formation of a cationic microblock structure in TPAA. In addition, TPAA displayed favorable thermal decomposition properties and a rough and coarse surface morphology, as shown by analyses using TGA and SEM, respectively. Moreover, a zip (type I) template polymerization mechanism was identified via analyses of the association constant (K_M), conversion (C_v) and polymerization rate (R_p). The flocculation performance of the templated copolymer TPAA was evaluated by treating high-turbidity water. According to the results for the zeta potentials and FTIR spectra of the generated flocs, it was indicated that the cationic microblocks in the templated copolymer could greatly enhance its charge neutralization, patching and bridging ability, and therefore excellent flocculation performance (residual turbidity: 5.8 NTU, D_f: 1.89, floc size d₅₀: 608.404 μm and floc kinetic: 15.86 × 10⁻⁴ s⁻¹) for treating high-turbidity water was achieved.