Mitochondrial and nuclear DNA dual damage induced by 2-(2′-quinolyl)benzimidazole copper complexes with potential anticancer activity

Jin'an Zhao; Shuangcheng Zhi; Huaibin Yu; Ruina Mao; Jiyong Hu; Wei Song; Junshuai Zhang

doi:10.1039/C7RA09102H

Mitochondrial and nuclear DNA dual damage induced by 2-(2′-quinolyl)benzimidazole copper complexes with potential anticancer activity†

Jin'an Zhao,

*^a Shuangcheng Zhi,^b Huaibin Yu,^c Ruina Mao,^c Jiyong Hu,*^a Wei Song^b and Junshuai Zhang^a

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* Corresponding authors

^a College of Chemical and Material Engineering, Henan University of Urban Construction, Pingdingshan 467036, Henan, P. R. China
E-mail: zjinan@zzu.edu.cn
Fax: +86-375-2089090
Tel: +86-375-2089090

^b School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan 467036, Henan, P. R. China

^c College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, Henan, P. R. China

Abstract

Two copper complexes, [Cu(qbm)Cl₂]₂ (1) and [Cu(qbm)Br(PPh₃)] (2) (where qbm = 2-(2′-quinolyl)benzimidazole), have been prepared and characterized. The interactions of both complexes with calf thymus DNA (CT-DNA) were detected by absorbance and emission spectroscopy methods; the results showed that both complexes were bound to CT-DNA via an intercalative mode. In addition, the two complexes significantly exhibited the free radicals dependence of DNA cleavage activity. MTT assays revealed that complex 2 showed good cell proliferation inhibitory activity against four different human cancer cell lines (SMMC7721, BGC823, HCT116 and HT29), particularly with HCT116 cells. Furthermore, morphological changes and flow cytometry analyses indicated that complex 2 can induce HCT116 cell death by apoptosis. These findings should promote the development of metal-based complexes for use as novel chemotherapeutic agents.

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DOI: https://doi.org/10.1039/C7RA09102H
Article type: Paper
Submitted: 17 Aug 2017
Accepted: 20 Oct 2017
First published: 03 Nov 2017
This article is Open Access

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RSC Adv., 2017,7, 51162-51174

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Mitochondrial and nuclear DNA dual damage induced by 2-(2′-quinolyl)benzimidazole copper complexes with potential anticancer activity

J. Zhao, S. Zhi, H. Yu, R. Mao, J. Hu, W. Song and J. Zhang, RSC Adv., 2017, 7, 51162 DOI: 10.1039/C7RA09102H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Mitochondrial and nuclear DNA dual damage induced by 2-(2′-quinolyl)benzimidazole copper complexes with potential anticancer activity†

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Mitochondrial and nuclear DNA dual damage induced by 2-(2′-quinolyl)benzimidazole copper complexes with potential anticancer activity

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