Issue 55, 2016, Issue in Progress
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RSC Advances

PEG–MWCNT/Fe hybrids as multi-modal contrast agents for MRI and optical imaging†

Anna Baranowska-Korczyc,*a   Małgorzata Jasiurkowska-Delaporte,*a   Barbara M. Maciejewska,ab   Alicja Warowicka,a   L. Emerson Coy,a   Tomasz Zalewski,a   Krzysztof K. Koziołc  and  Stefan Jurgaab  

* Corresponding authors

a NanoBioMedical Centre, Adam Mickiewicz University, ul. Umultowska 85, PL-61614 Poznań, Poland
E-mail: akorczyc@amu.edu.pl, maljas@amu.edu.pl

b Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, PL-61614 Poznań, Poland

c University of Cambridge, Department of Materials Science and Metallurgy, 27 Charles Babbage Road, Cambridge CB3 0FS, UK

Abstract

This study examines the use of oxidized multi-walled carbon nanotube/iron (O-MWCNT/Fe) nanohybrids modified with polyethylene glycol (PEG) as multifunctional cellular imaging agents for magnetic resonance imaging (MRI) and fluorescence microscopy. The PEGylated MWCNTs with embedded iron particles were investigated as T2-weighted contrast agents for MRI. The number of PEG molecules attached to the MWCNT surface was calculated. The PEG–MWCNT/Fe complex was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared and Raman spectroscopies. Covalent surface modification of the MWCNTs improves their solubility and enables the attachment of further biomolecules to their surface. The PEGylated nanostructures were labeled with the MDC organic dye and internalized inside HeLa cells for cellular imaging. Additionally, the minimal cytotoxic effect of PEGylated complexes in comparison to non-PEGylated samples was measured using the WST-1 test and an In Cell Analyzer. A confocal microscopy study of the organelle morphology also confirmed that the HeLa cell morphology was unchanged after treatment with PEGylated MWCNTs.

Graphical abstract: PEG–MWCNT/Fe hybrids as multi-modal contrast agents for MRI and optical imaging

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Article information

DOI
https://doi.org/10.1039/C6RA09191A
Article type
Paper
Submitted
09 Apr 2016
Accepted
12 May 2016
First published
16 May 2016

RSC Adv., 2016,6, 49891-49902

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PEG–MWCNT/Fe hybrids as multi-modal contrast agents for MRI and optical imaging

A. Baranowska-Korczyc, M. Jasiurkowska-Delaporte, B. M. Maciejewska, A. Warowicka, L. E. Coy, T. Zalewski, K. K. Kozioł and S. Jurga, RSC Adv., 2016, 6, 49891 DOI: 10.1039/C6RA09191A

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