Issue 93, 2014
From the journal:

RSC Advances

Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells†

Nadezhda M. Zholobak,a   Alexander B. Shcherbakov,a   Ekaterina O. Vitukova,b   Alla V. Yegorova,b   Yulia V. Scripinets,b   Inna I. Leonenko,b   Alexander Ye. Baranchikov,c   Valeriy P. Antonovichb  and  Vladimir K. Ivanov*cd  

* Corresponding authors

a Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv D0368, Ukraine

b Bogatsky Physicochemical Institute, National Academy of Sciences of Ukraine, Odessa, Ukraine

c Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
E-mail: van@igic.ras.ru

d National Research Tomsk State University, Tomsk 634050, Russia

Abstract

A method for the direct monitoring of the interaction of reactive oxygen species (ROS) with cerium dioxide nanoparticles (CDN) in living cells is proposed based on the use of a complex of calcein and CDN. The CDN–calcein complex penetrates easily into a living cell and is decomposed readily by endogenous or exogenous ROS, releasing brightly fluorescent calcein, which can be observed using conventional fluorescent microscopy. This complex is less cytotoxic than individual cerium dioxide nanoparticles and provides effective protection from oxidative stress induced by the action of hydrogen peroxide, treatment with latex beads, or infection by the vesicular stomatitis virus.

Graphical abstract: Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4RA08292C
Article type
Paper
Submitted
07 Aug 2014
Accepted
22 Sep 2014
First published
22 Sep 2014

RSC Adv., 2014,4, 51703-51710

Permissions

Request permissions

Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

N. M. Zholobak, A. B. Shcherbakov, E. O. Vitukova, A. V. Yegorova, Y. V. Scripinets, I. I. Leonenko, A. Ye. Baranchikov, V. P. Antonovich and V. K. Ivanov, RSC Adv., 2014, 4, 51703 DOI: 10.1039/C4RA08292C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements