Antineoplastic activity of strawberry (Fragaria × ananassa Duch.) crude extracts on B16-F10 melanoma cells

Cinzia Forni; Roberto Braglia; Nadia Mulinacci; Andrea Urbani; Maurizio Ronci; Angelo Gismondi; Claudio Tabolacci; Bruno Provenzano; Alessandro Lentini; Simone Beninati

doi:10.1039/C3MB70316A

Antineoplastic activity of strawberry (Fragaria × ananassa Duch.) crude extracts on B16-F10 melanoma cells

Cinzia Forni,*^a Roberto Braglia,^a Nadia Mulinacci,^b Andrea Urbani,^c Maurizio Ronci,^cd Angelo Gismondi,^a Claudio Tabolacci,^a Bruno Provenzano,^a Alessandro Lentini^a and Simone Beninati^a

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^a Department of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, Italy
E-mail: forni@uniroma2.it
Fax: +39 062023500
Tel: +39 0672594345

^b Department of Neurosciences, Psychology, Drug Research and Child Health, Division of Pharmaceutical and Nutraceutical Sciences and Multidisciplinary Centre of Research on Food Sciences (M.C.R.F.S.-Ce.R.A.), University of Florence, Sesto Fiorentino, Florence, Italy

^c European Brain Research Institute (EBRI), IRCCS Fondazione Santa Lucia, Rome, Italy

^d Mawson Institute, University of South Australia, Mawson Lakes SA 5095, Australia

Abstract

The antiproliferative and differentiation potential of anthocyanin-rich strawberry fruit crude extracts (SE) were investigated on B16-F10 murine melanoma cells. Treatment of melanoma cells with SE produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, and the enhancement of tissue transglutaminase (TG2, EC 2.3.2.13) activity (used as a differentiation marker). To gain further insight into profiling altered protein expression as a potential biomarker of the SE action on melanoma cells, analysis of the proteomic profile was performed on the treated B16-F10 cells, compared to the control. Following SE treatment, 30 proteins resulted up-regulated, and 87 proteins were down-regulated. In particular proteins overexpressed in cancer cells, involved in tumor progression and metabolism, were down-regulated. The possibility that SE may affect the Warburg effect in B16-F10 melanoma cells is discussed.

This article is part of the themed collection: Italian Proteomics Association, 2014

Molecular BioSystems

Antineoplastic activity of strawberry (Fragaria × ananassa Duch.) crude extracts on B16-F10 melanoma cells

Abstract

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Antineoplastic activity of strawberry (Fragaria × ananassa Duch.) crude extracts on B16-F10 melanoma cells

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