Issue 4, 2013
From the journal:

Journal of Materials Chemistry B

Apoptosis induction and inhibition of drug resistant tumor growth in vivo involving daunorubicin-loaded graphene–gold composites†

Gen Zhang,ad   Hucheng Chang,a   Christian Amatore,b   Yu Chen,c   Hui Jianga  and  Xuemei Wang*a  

* Corresponding authors

a State Key Laboratory of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
E-mail: xuewang@seu.edu.cn
Tel: +86 025-83792177

b Ecole Normale Supérieure, UMR CNRS-ENS-UPMC 8640 and LIA CNRS XiamENS NanoBioChem Departement de Chimie, 24 rue Lhomond 75005 Paris, France

c Key Laboratory for Advanced Materials, Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

d Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, China

Abstract

Daunorubicin (DNR) loaded graphene–gold nanocomposites offer a novel strategy for inducing apoptosis in drug resistant leukemia cells (K562/A02; KA). In vitro and in vivo investigations on xenografted tumors in KA nude mice demonstrate that the combination of monoclonal P-glycoprotein (P-gp) antibodies and DNR anticancer drug loaded on graphene–gold nanocomposites (GGN) is an efficient drug delivery vector, with remarkable targeting and binding properties towards drug resistant KA cell lines, and induces apoptosis of KA cells and inhibits tumor growth in KA nude mice. Cellular treatment with DNR-loaded GGN remarkably reduced drug resistant-related P-gp expression and activated apoptosis-related caspase protein expression in KA cells. Cell apoptosis provoked in vitro by such nanocomposites corresponds to a rapid induction of active caspase 8,3 activities and stimulation of poly-(ADP-ribose) polymerase (PARP) proteolytic cleavage. In vivo studies indicate that DNR-loaded GGN nanocomposites effectively overcome the inhibition of drug resistant leukemia cell-induced tumor growth in KA nude mice. This nanocomposite raises the possibility of modulating apoptosis in cancer cells, and of inhibiting tumor growth, showing that nanocomposites of this kind have promising applications in efficient multifunctional therapy.

Graphical abstract: Apoptosis induction and inhibition of drug resistant tumor growth in vivo involving daunorubicin-loaded graphene–gold composites

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2TB00378C
Article type
Paper
Submitted
07 Nov 2012
Accepted
08 Nov 2012
First published
19 Nov 2012

J. Mater. Chem. B, 2013,1, 493-499

Permissions

Request permissions

Apoptosis induction and inhibition of drug resistant tumor growth in vivo involving daunorubicin-loaded graphene–gold composites

G. Zhang, H. Chang, C. Amatore, Y. Chen, H. Jiang and X. Wang, J. Mater. Chem. B, 2013, 1, 493 DOI: 10.1039/C2TB00378C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements