Issue 10, 2012

Design of protein congeners containing β-cyclopropylalanine

Abstract

The non-canonical amino acid (ncAA) analogue of methionine (Met), β-cyclopropylalanine (Cpa), was successfully incorporated into recombinant proteins expressed in Escherichia coli in a residue-specific manner. Proteins substituted in this way are congeners because they derive from the same gene sequence as the parent protein but contain a fraction of ncAAs. We have expressed congeners using parent and mutant gene sequences of various proteins (lipase, annexin A5, enhanced green fluorescent protein, and barstar) and found that Cpa incorporation is highly dependent on the protein sequence composition. These results indicate that the global amino acid composition of proteins might be a crucial parameter that influences the outcome of unnatural translation. In addition, we could also demonstrate that the chemical nature of the second residue could be essential for successful ncAA incorporation.

Graphical abstract: Design of protein congeners containing β-cyclopropylalanine

Supplementary files

Article information

Article type
Paper
Submitted
17 May 2012
Accepted
12 Jul 2012
First published
13 Jul 2012

Mol. BioSyst., 2012,8, 2719-2723

Design of protein congeners containing β-cyclopropylalanine

C. G. Acevedo-Rocha, A. Geiermann, N. Budisa and L. Merkel, Mol. BioSyst., 2012, 8, 2719 DOI: 10.1039/C2MB25193K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements