Issue 1, 2010
From the journal:

Lab on a Chip

A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber†

Thomas M. Keenan,*a   Charles W. Frevert,bc   Aileen Wu,d   Venus Wongbc  and  Albert Folchd  

* Corresponding authors

a Stem Cell and Regenerative Medicine Center, 1111 Highland Ave, Madison, WI, USA
E-mail: tkeenan@wisc.edu
Tel: +1 608-262-7331

b Department of Medicine, University of Washington, Seattle, WA, USA

c Medical Research Service, Veterans Affairs Medical Center, Seattle, WA, USA

d Department of Bioengineering, University of Washington, Campus Box 355061, Seattle, WA, USA
E-mail: afolch@u.washington.edu
Tel: +1 206-685-2257

Abstract

During inflammation neutrophils rapidly migrate to the site of tissue damage or infection by following complex gradients of bacterial peptides and host-derived chemokines. The efficiency and speed of neutrophil migration is critically dependent upon the ability of neutrophils to sense new gradients and utilize only those that provide the most direct path to the damaged or infected site. Receptor desensitization plays an important role in migration efficiency and is most commonly studied using bath application of chemotactic factor solutions instead of presenting cells with gradients analogous to those they would experience in vivo. Here we describe a new method for examining gradient-induced neutrophil desensitization using a previously-developed open-chamber microfluidic gradient generator.

Graphical abstract: A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber

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Article information

DOI
https://doi.org/10.1039/B913494H
Article type
Method
Submitted
09 Jul 2009
Accepted
02 Oct 2009
First published
03 Nov 2009

Lab Chip, 2010,10, 116-122

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A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber

T. M. Keenan, C. W. Frevert, A. Wu, V. Wong and A. Folch, Lab Chip, 2010, 10, 116 DOI: 10.1039/B913494H

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