SCHEDULED MAINTENANCE
Separation of soluble selenium compounds in muscle from seven animal species using size exclusion chromatography and inductively coupled plasma mass spectrometry†
Abstract
To study the role of selenium compounds for the quality and nutritional value of meat, speciation of selenium compounds was performed in two muscles from each of seven animal species (chicken, turkey, duck, ostrich, lamb, cattle and pig). Soluble selenium compounds were separated by size-exclusion chromatography (SEC) and detected by inductively coupled plasma-mass spectrometry (ICP-MS). Four selenium peaks were found in the muscle extracts by SEC, with partition coefficients (Kav) of 0.02, 0.44, 0.74 and 0.86, respectively. The second and third peaks had a chromatographic mobility approximately corresponding to that of tetrameric glutathione peroxidase and selenoprotein W, respectively, and they contained 68–100% of the recovered selenium in different muscles. The distribution of selenium among the four peaks varied considerably in muscles from different species, the second peak accounting for 28–71% of the recovered selenium and the third peak for 17–72%. These differences in selenium distribution among animal species are discussed in relation to meat quality and nutritional value.
Please wait while we load your content...
