Abstract
Constitutive androstane receptor (CAR) regulates hepatic xenobiotic and energy metabolism, as well as promotes cell growth and hepatocarcinogenesis. Berberine is an ancient multipotent alkaloid drug which derived from Coptis chinensis plants. Here we report that berberine is able to be cellular uptake and accessible to chromatin in human hepatoma HepG2 cells. Berberine induces more apoptosis, cell cycle arrest, but less ROS production in CAR overexpressed mCAR-HepG2 cells. Moreover, berberine inhibits expressions of CAR and its target genes CYP2B6 and CYP3A4. Furthermore, berberine enhances DNA methylation level in whole genome but reduces that in promoter regions CpG sites of CYP2B6 and CYP3A4 genes under the presence of CAR condition. These results indicated that the antiproliferation of berberine might be mediated by the unique epigenetic modifying mechanism of CAR metabolic pathway, suggesting that berberine is a promising candidate in anticancer adjuvant chemotherapy, due to its distinct pharmacological properties in clinic.
Similar content being viewed by others
Introduction
Constitutive androstane receptor (CAR) is a transcription factor which plays an important role in the hepatic exogenous and endogenous metabolisms by regulating the expression of its target genes1,2. In the presence of activators such as phenobarbital (PB), CAR is dissociated from its co-chaperone partners and translocate into the nucleus3,4. Following the dimerizing with the retinoid X receptor (RXR) then binding to PB responsive enhancer modules (PBREM) in its target promoters, the expressions of many metabolic target genes are transcriptional activated5. CAR regulates numerous genes encoding drug- and xenobiotic- metabolizing enzymes, such as cytochrome P450 2B6 (CYP2B6) and CYP3A4 isoenzymes6. CAR also influences endobiotic energy metabolism, including lipogenesis, fatty acids oxidation and glucose homeostasis7. Recently research showed that CAR promotes multiple tumor proliferation and metastasis and induces resistance for antitumor chemotherapeutics8,9,10. Therefore, targeting CAR metabolic pathway has been considered a novel anticancer drug development approach.
Berberine is an isoquinoline alkaloid derived from Coptis chinensis plants11. Berberine-containing plants (Huanglian) have been used to treat diarrhea, gastroenteritis, feverous and hepatic disorders in traditional Chinese medicines for centuries12,13. Pharmacological effects of berberine including anti-inflammatory, hypoglycemic, hypolipidemic and neuroprotection have been well demonstrated14,15,16. As an over the counter (OTC) medicine in China with low reported side effect, berberine is also widely used in clinic to treat dysentery, type 2 diabetes mellitus, hypertension, hypercholesterolemia and neurodegenerative disorders17. Recent studies have proved that berberine can inhibits proliferation of multiple human tumor cells18,19. However, whether the antitumor effect of berberine is associated with CAR metabolizing pathway remains unclear.
DNA methylation is one of the most common epigenetic modification mechanisms in gene expression, which often exhibits as changes of 5-methylcytosine level in CpG dinucleotide (CpG-island) located in the promoters of many genes or chromatins20. Abnormal DNA methylation including global genome hypomethylation is caused by oncogenes activation and chromosomal instability, while hypermethylation in GpG-rich promoter regions results in silencing of tumor suppressor genes in hepatocarcinogenesis21,22,23,24,25. The biotransformation and disposition of berberine is mainly oxidative demethylation and demethylenation by hepatic phase I drug metabolic CYP450 isoenzyme and subsequent glucuronidation to facilitate excretion26,27,28. The metabolites of berberine are remained the pharmacological activities in spite of potency. However, the pharmacological mechanism of berberine such as whether it involves epigenetic modification of CAR and the target genes CYP2B6 and CYP3A4 metabolic pathway remains unknown. The current research investigated whether berberine could be intracellular uptake and accessible to chromatin, inhibit proliferation and influence DNA methylation states of CAR and its target gene CYP2B6 and CYP3A4 pathway in human hepatoma HepG2 cells.
Results and Discussion
Intracellular distribution and anti-proliferation of berberine in HepG2 cells
It has been reported that in human transformed and immortalized cells, such as hepatoma HepG2 cells, lack the capability of retaining CAR in the cytoplasm, so that CAR spontaneously accumulates in the nucleus, while in mouse hepatocytes CAR is expressed predominantly in the cytoplasm before activation29,30,31,32,33. In order to investigate whether CAR is involved in the effect of berberine on cell proliferation, the nucleocytoplasmic localization of CAR was evaluated in wild-type and mouse CAR stably transfected HepG2 cells (mCAR-HepG2 cells), using the confocal laser scanning microscopy (CLSM) imaging method (Fig. 1a). As expected, compared with the HepG2 cells, the green fluorescence of CAR were clearly enhanced in mCAR-HepG2 cells. Moreover, the fluorescence intensities of CAR were higher in both cytoplasma and nucleus in the mCAR-HepG2 cells than that in the HepG2 cells (Fig. 1b).
Berberine exhibits disconnection between its excellent pharmacodynamics and poor pharmacokinetic properties both in vitro and in vivo34,35. Moreover, the main methods currently used to detect berberine in plasma concentration and tissues disposition are high performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrum (LC-MS)36,37,38,39. To better understand berberine’s antiproliferation activity in hepatoma cells, we investigated the cellular uptake, distribution and localization of berberine utilizing its fluorescent molecular properties40,41, by both of flow cytometry and CLSM methods. As shown in Fig. 1c, the total cellular uptake of berberine was significantly increased in a concentration-dependent pattern (5, 10 and 25 μM), detected by the flow cytometer for 24 h treatment. However, the differences in cellular uptake of berberine were not observed between HepG2 cells and mCAR-HepG2 cells (Fig. 1d), suggesting CAR does not influence the uptake or efflux of berberine in HepG2 cells.
Furthermore, the intracellular distribution of berberine was observed in both HepG2 cells and mCAR-HepG2 cells by CLSM (Fig. 1e). Compared with control groups (DMSO), yellow fluorescent was detected in cytoplasm at the lower incubation concentration (1 μM). Under the 5 μM condition, distribution of berberine was more clearly observed in cytoplasm and around nucleus. At a higher concentration (25 μM), more fluorescence of berberine was determined in both of cytoplasm and nucleus. These results indicated that berberine was able to be cellular uptake even under lower concentration, then the intracellular distribution of berberine was gradually accumulated and located from cytoplasm into nucleus and reached chromatin by the concentration-dependent manner in the range of 1 μM to 25 μM in HepG2 cells (Fig. 1f) and mCAR-HepG2 cells (Fig. 1g). The results also suggested that berberine might have selective impact on the proliferation of HepG2 cells and mCAR-HepG2 cells.
Effects of berberine on the cells growth and viability were evaluated by alamar blue assay. After co-incubation with different concentrations of berberine for 24 h (Fig. 1h) or 48 h (Fig. 1i), the significant differences of cell viabilities were not observed at lower concentrations of berberine (from 0.1 μM to 20 μM) in both HepG2 cells and mCAR-HepG2 cells. However, in the presence of higher concentration (50 and 100 μM), both the HepG2 cells and mCAR-HepG2 cells viabilities were significantly inhibited after incubation for 24 h and 48 h. Moreover, the cell proliferation was more significantly inhibited by berberine in mCAR-HepG2 cells than that in HepG2 cells after both 24 h and 48 h treatment (P < 0.01). These results supported the previous observation about berberine had higher affinity with nucleus and chromatin42,43 and suggested that the endonuclear DNA might be a target of anti-proliferating pharmacodynamics induced by berberine.
Berberine induces apoptosis, cell cycle arrest and ROS production in absence or presence of mCAR in HepG2 cells
To confirm whether inhibition of proliferation by berberine has impact on cancer cell survival, the percentage of apoptotic cells were counted with annexin V/propidium iodide (PI) double fluorescent staining by flow cytometry. The total apoptosis including early apoptotic cells were shown in the lower right quadrant of the scatter plot and necrotic cells in the upper right quadrant. Berberine induced the apoptosis in a dose- and time- dependent manner in the range of 1, 5 and 25 μM after treated with berberine for 24 h (Fig. 2a,b) and 48 h (Fig. 2c,d). Similar to the results of cell viability/proliferation, the rates of the apoptosis induced by berberine in mCAR-HepG2 cells were significantly higher than that in the HepG2 cells. These results are consistent with previous reports that berberine induces apoptosis in multiple human cancer cells44,45, suggesting that the inhibition of proliferation by berberine might be mediated by induction of apoptosis.
Furthermore, the effects of berberine on the cell cycle progression were detected using prodium iodide (PI) probes and analyzed with flow cytometry. Compared with 24 h time point (Fig. 2e–g), berberine promoted cell cycle arrest at G0/G1 phase, conversely, the number of cells in the S phase were decreased accordingly by berberine in the range of 1, 5, 25, 50 μM concentrations after treatment of 48 h (Fig. 2h–j). Similar to the above results of antiproliferation and inducing apoptosis, berberine-enhanced percentage of cell cycle arrest at G0/G1 phase were higher in mCAR-HepG2 cells than that in HepG2 cells, suggesting that the inhibitory effects of berberine on the mCAR-HepG2 cells proliferation were more sensitivity than that in HepG2 cells.
Reactive oxygen species (ROS) has been reported to mediate oxidative damage in berberine induced apoptosis, necrosis and inhibition of proliferation in several types of tumor cells46,47. Therefore, the present research investigated whether berberine induced more ROS production in mCAR-HepG2 than that in HepG2 cells. The ROS production was determined using dihydroethidium, as a cellular fluorescence dye analyzed by flow cytometry. The present results showed that berberine increased ROS production in the range of 1, 5, 25, 50 μM at concentration-dependent manner in both HepG2 cells (Fig. 2k) and mCAR-HepG2 cells (Fig. 2l). Unexpectedly, berberine-induced ROS production in mCAR-HepG2 cells was less than that in HepG2 cells, suggesting that there might be both ROS-dependent and ROS-independent mechanisms of mCAR-HepG2 cells death stimulated by berberine (Fig. 2m). Because the cytotoxicity-induced by ROS is also recognized as one of the cause of side effects of anti-tumor chemotherapy48, the ROS-independent mechanism induced by berberine might be more beneficial for chemotherapy in clinic.
Berberine inhibits intracellular accumulation of CAR and suppresses expressions of CYP2B6 and CYP3A4 mRNA
In our previous study, the effect of berberine on the early phase of hepatocarcinogenesis stimulated by diethylnitrosamine (DEN) plus phenobarbital (PB) in rats was investigated in vivo. We found that oral administration of berberine (50 mg/kg) alleviated hepatomegaly, inhibited the hepatocyte proliferating cell nuclear antigen (PCNA) expressions, decreased cytochrome P450 content and inhibited CYP2E1 and CYP1A2 activities in DEN-plus-PB treated rats49. Moreover, several researchers also reported that berberine inhibited the expression and activity of CYP450 enzymes in vivo and in vitro50. The present study further investigated whether berberine affects the upstream transcription factors on CYP450 expression, especially nuclear receptor CAR, in the subcellular location determined with CLSM in the mCAR-HepG2 cells. The present result showed that berberine significantly inhibited the accumulation of CAR protein (Fig. 3a,b), the nucleoplasmic retention ratios (Fig. 3c), expression of CAR mRNA (Fig. 3d) and protein (Fig. 3e) in mCAR-HepG2 cells in the range of 1 to 25 μM at a concentration-dependent manner.
In the absence of berberine (DMSO group), a difference in expression of CYP3A4, CYP2B6 and glucose regulated protein 78 (GRP78) genes were observed between HepG2 (Fig. 3f) and mCAR-HepG2 cells (Fig. 3g). The expressive level of CYP2B6 gene was higher than that of GRP78 and CP3A4 in mCAR-HepG2 cells, whereas CYP3A4 and GRP78 were higher in HepG2 cells. Compared with control (DMSO), berberine markedly inhibited the expression of CYP2B6 mRNA in mCAR-HepG2 cells (P < 0.05). However, berberine seemed to enhance the expression of CYP2B6 mRNA in HepG2 cells (Fig. 3h). Moreover, berberine inhibited expression of CYP3A4 in mCAR-HepG2 and HepG2 cells in the dose-dependent manner (1, 5, 25 μM) (Fig. 3i). Similar to the berberine effect on ROS production described above, the present result showed that berberine-enhanced expression levels of GRP78 mRNA (Fig. 3j) in mCAR-HepG2 cells were lower than that in HepG2 cells (P < 0.05). GRP78 is not only considered as one of chaperones with CAR-shuttling between cytoplasm and nucleus, it is also accepted as one of the biomarkers in endoplasmic reticulum stress51. Therefore, this result further supported that the non-oxidative stress dependent mechanism likely involve in the berberine induced inhibition of proliferation in mCAR-HepG2 cells.
Berberine alters DNA methylation status of whole genome and promoter region CpG sites in CYP2B6 and CYP3A4 genes
As shown in Fig. 4a, there are three major demethylation metabolic sites have been confirmed in the molecular structure of berberine52. Then, this research investigated whether the inhibition of berberine on the gene expression is associated with the methylation status change of CAR target gene. As shown in Fig. 4b, the global DNA methylation levels were enhanced by berberine in the range from 5 to 25 μM, in both of HepG2 and mCAR-HepG2 cells. These results supported the hypothesis that demethylated metabolism of berberine is likely to cause the genomic DNA methylation, hence enhances the global methylation level and increase the chromosomal stability of metabolic enzymes20.
It has been reported that the methylation of CpG residues in promoter region might be provided the molecular docking sites and sequentially recruited transcription factor to modify chromatin and regulate gene expression53. Thus, the methylation levels of total eight individual and average CpG sites (5-methylation of cytosine basic group) in CYP2B6 proximal promoter region were analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) method. As shown in Fig. 4c,d, the methylation levels of every individual and average CpG sites in CYP2B6 gene promoter in mCAR-HepG2 cells, were significantly higher than that in HepG2 cells, except at the 6 th and 8 th CpG sites for which the signal were not detected (P < 0.05). This result supported that CAR induce the hypermethylation in CYP2B6 promoter region and leads for inhibition of transcription of anti-oncogenes, then causing tumor cellular proliferation54. On the other hand, berberine significantly reduced the hypermethylation status of individual and average CpG sites in CYP2B6 promoter in mCAR-HepG2 cells (P < 0.05) (Fig. 4e), suggesting that the epigenetic modifying of berberine on the CYP2B6 promoter might be an early event and likely involved in the inhibition mechanism of CYP2B6 gene expression in mCAR-HepG2 cells.
Similar to the effect of berberine on CYP2B6 gene, the methylation levels of two individual CpG sites (CpG 2nd and CpG 3rd sites) and average CpG sites within CYP3A4 gene promoter region in mCAR-HepG2 cells were higher than that in HepG2 cells (Fig. 4f,g). However, the significantly decrease of average methylation level induced by berberine (5 μM) were not observed in all CpG sites within CYP3A4 gene promoter region (Fig. 4h). These results suggested that inhibition mechanism of berberine on CYP3A4 gene expression might be involved in enhancing methylation level of global genome, rather than the hypermethylation in the promoter region induced by CAR in HepG2 cells. Furthermore, regardless of absence or presence of berberine (10 μM), the significantly difference were not observed in methylation levels of individual and average CpG sites within GRP78 gene promoter region between HepG2 and mCAR-HepG2 cells (Fig. 4i–k).
Conclusion
Our research showed that berberine is able to be cellular uptake and accessible to chromatin in human hepatoma HepG2 cells. Berberine induces more apoptosis, cell cycle arrest, but less ROS production in mCAR-HepG2 cells than that in HepG2 cells. Moreover, berberine reduced intracellular accumulation of CAR, suppressed gene expression of CYP2B6 and CYP3A4 metabolic enzymes. Furthermore, berberine enhances DNA methylation level in whole genome but reduces that in promoter regions of CYP2B6 and CYP3A4 genes under the presence of CAR condition. These results indicated that the anti-proliferative effect of berberine might be mediated, at least partially by the unique epigenetic modifying mechanism of CAR metabolic pathway, suggesting that berberine is a promising candidate drug in anticancer adjuvant chemotherapy, with its distinct pharmacological properties and already demonstrated safety in clinic (Fig. 5).
Materials and Methods
A full explanation of all methods used can be found within the Supplemental Information.
Plasmid, stable transfection and cell culture condition
Human hepatoma HepG2 cells were obtained from the American Type Culture Collection (ATCC, USA). The plasmid pCR3-mCAR expressing mouse CAR cDNA was constructed and kindly provided by Professor Hong-Bing Wang (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, USA)55,56. Plasmids were stably transfected into HepG2 cells (mCAR-HepG2 cell line) by LipofectamineTM 2000 according to the manufacturer’s guidelines. (See also supplementary materials and methods).
Confocal laser scanning microscopy (CLSM) imaging
Both intracellular distribution of berberine utilizing its fluorescent molecular properties and subcellular location of constitutive androstane receptor (CAR) were determined by confocal laser scanning microscopy (CLSM, Leica TCS SP5, Heidelberg, Germany) imaging method.
Flow cytometry
Detections of cell cycle, apoptosis, reactive oxygen species (ROS) and intracellular distribution of berberine were performed using BD FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Assessment of cell viability/proliferation
Assessment of cell viability/proliferation was performed by commercial Alamar Blue kit obtained from SunBio Co. (Shanghai, China) at 570 nm and 620 nm (OD570-OD620) by a spectrophotometer Multiskan MK3 (Thermo Fisher Scientific, USA).
RNA extraction and quantitative real-time PCR (qRT-PCR)
Total RNA was isolated from cultured cells using RNApure kit (BioTeke, Beijing, China) according to the manufacturer’s instructions. RNA purity was determined from the ratio of optical density value (OD260/280) in range of 1.8–2.1. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with a Stratagene Mx3005P QPCR system (Agilent, Biosystems, Forest City, CA, USA). (See also supplementary materials and methods).
DNA extraction and global DNA methylation analyses
Genomic DNA was extracted from cultured cells with NucleoSpin Tissue kit (MACHEREY-NAGEL, Germany) according to manufacturer’s instructions. Global genomic DNA methylation was detected with the Methylamp Global DNA Methylation Quantification Ultra Kit (Epigentek, New York, NY) according to manufacturer’s instructions. The methylated fractions of DNA are recognized by an anti-5-methylcytosine antibody and quantified by an enzyme-linked immunosorbent assay-like reaction.
Methylation analysis in promoter region by MassARRAY platform
Specific DNA methylation status of CYP2B6, CYP3A4 and GRP78 promoter was estimated via the Sequenom MassARRAY platform (CapitalBio, Beijing, China). The procedure of quantitative DNA methylation analysis of CYP2B6, CYP3A4 and GRP78 genes in promoter regions included bisulfite treatment of DNA, PCR amplification, in vitro transcription, RNA base-specific cleavage and matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF-MS) analysis (MassARRAY Analyzer 4 system, Sequenom, San Diego, CA, USA)57,58. (See also supplementary materials and methods).
Statistical analysis
Results are presented as mean ± standard deviation (SD) and analyzed by SPSS software (version 16.0). Statistical significance of mean values between multiple treatment groups was accessed by one-way analysis of variance (ANOVA). P value < 0.05 was considered statistically significant.
Additional Information
How to cite this article: Zhang, L. et al. Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells. Sci. Rep. 6, 28116; doi: 10.1038/srep28116 (2016).
References
Wei, P., Zhang, J., Egan-Hafley, M., Liang, S. & Moore, D. D. The nuclear receptor CAR mediates specific xenobiotic induction of drug metabolism. Nature 407, 920–923 (2000).
Xie, W. et al. Humanized xenobiotic response in mice expressing nuclear receptor SXR. Nature 406, 435–439 (2000).
Mutoh, S. et al. Phenobarbital indirectly activates the constitutive active androstane receptor (CAR) by inhibition of epidermal growth factor receptor signaling. Sci. Signal 6, ra31 (2013).
Xu, R. X. et al. A structural basis for constitutive activity in the human CAR/RXR alpha heterodimer. Mol. Cell 16, 919–928 (2004).
Suino, K. et al. The nuclear xenobiotic receptor CAR: structural determinants of constitutive activation and heterodimerization. Mol. Cell 16, 893–905 (2004).
Swales, K. & Negishi, M. CAR, driving into the future. Mol. Endocrinol. 18, 1589–1598 (2004).
Wada, T., Gao, J. & Xie, W. PXR and CAR in energy metabolism. Trends Endocrinol. Metab. 20, 273–279 (2009).
Yang, H. & Wang, H. Signaling control of the constitutive androstane receptor (CAR). Protein Cell 5, 113–123 (2014).
Nebert, D. W. & Dalton, T. P. The role of cytochrome P450 enzymes in endogenous signalling pathways and environmental carcinogenesis. Nat. Rev. Cancer 6, 947–960 (2006).
Yamamoto, Y., Moore, R., Goldsworthy, T. L., Negishi, M. & Maronpot, R. R. The orphan nuclear receptor constitutive active/androstane receptor is essential for liver tumor promotion by phenobarbital in mice. Cancer Res. 64, 7197–7200 (2004).
Singh, I. P. & Mahajan, S. Berberine and its derivatives: a patent review (2009–2012). Expert Opin. Ther. Pat. 23, 215–231 (2013).
Menees, S., Saad, R. & Chey, W. D. Agents that act luminally to treat diarrhoea and constipation. Nat. Rev. Gastroenterol. Hepatol. 9, 661–674 (2012).
Tillhon, M., Guamán Ortiz, L. M., Lombardi, P. & Scovassi, A. I. Berberine: New perspectives for old remedies. Biochem. Pharmacol. 84, 1260–1267 (2012).
Kong, W. et al. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat. Med. 10, 1344–1351 (2004).
Bae, J. et al. Berberine protects 6-hydroxydopamine-induced human dopaminergic neuronal cell death through the induction of heme oxygenase-1. Mol. Cells 35, 151–157 (2013).
Gowans, G. J., Hawley, S. A., Ross, F. A. & Hardie, D. G. AMP is a true physiological regulator of AMP-activated protein kinase by both allosteric activation and enhancing net phosphorylation. Cell Metab. 18, 556–566 (2013).
Winkler, A. et al. A concerted mechanism for berberine bridge enzyme. Nat. Chem. Biol. 4, 739–741 (2008).
Tang, J. et al. Berberine and Coptidis Rhizoma as novel antineoplastic agents: A review of traditional use and biomedical investigations. J. Ethnopharmacol. 126, 5–17 (2009).
Diogo, C. V. et al. Berberine as a promising safe anti-cancer agent - is there a role for mitochondria? Curr. Drug Targets 12, 850–859 (2011).
Dawson, M. A. & Kouzarides, T. Cancer epigenetics: from mechanism to therapy. Cell 150, 12–27 (2012).
Mudbhary, R. et al. UHRF1 overexpression drives DNA hypomethylation and hepatocellular carcinoma. Cancer Cell 25, 196–209 (2014).
Bert, S. A. et al. Regional activation of the cancer genome by long-range epigenetic remodeling. Cancer Cell 23, 9–22 (2013).
Tantin, D. & Rutter, J. Proliferation and metabolism: it’s as easy as APC. Cell Metab. 15, 413–414 (2012).
Thompson, C. B. Metabolic enzymes as oncogenes or tumor suppressors. N. Engl. J. Med. 360, 813–815 (2009).
Hardie, D. G. Targeting the core of transcription. Science 329, 1158–1159 (2010).
He, S. M. et al. Disposition pathways and pharmacokinetics of herbal medicines in humans. Curr. Med. Chem. 17, 4072–4113 (2010).
Liu, Y. et al. Oxidative demethylenation and subsequent glucuronidation are the major metabolic pathways of berberine in rats. J. Pharm. Sci. 98, 4391–4401 (2009).
Li, X. L., Hu, Y. J., Wang, H., Yu, B. Q. & Yue, H. L. Molecular spectroscopy evidence of berberine binding to DNA: comparative binding and thermodynamic profile of intercalation. Biomacromolecules 13, 873–880 (2012).
Hosseinpour, F., Moore, R., Negishi, M. & Sueyoshi, T. Serine 202 Regulates the nuclear translocation of constitutive active/androstane receptor. Mol. Pharmacol. 69, 1095–1102 (2006).
Swales, K. et al. Novel CAR-mediated mechanism for synergistic activation of two distinct elements within the human cytochrome P450 2B6 gene in HepG2 cells. J. Biol. Chem. 280, 3458–3466 (2005).
Chen, Y., Ferguson, S. S., Negishi, M. & Goldstein, J. A. Identification of constitutive androstane receptor and glucocorticoid receptor binding sites in the CYP2C19 promoter. Mol. Pharmacol. 64, 316–324 (2003).
Mutoh, S. et al. Dephosphorylation of threonine 38 is required for nuclear translocation and activation of human xenobiotic receptor CAR (NR1I3). J. Biol. Chem. 284, 34785–34792 (2009).
Kamino, H. et al. Nuclear receptor CAR-regulated expression of the FAM84A gene during the development of mouse liver tumors. Int. J. Oncol. 38, 1511–1520 (2011).
Zhang, J. et al. Pharmacokinetics-pharmacology disconnection of herbal medicines and its potential solutions with cellular pharmacokinetic-pharmacodynamic strategy. Curr. Drug Metab. 13, 558–576 (2012).
Liu, Y. T. et al. Extensive intestinal first-pass elimination and predominant hepatic distribution of berberine explain its low plasma levels in rats. Drug Metab. Dispos. 38, 1779–1784 (2010).
Patil, S., Dash, R. P., Anandjiwala, S. & Nivsarkar, M. Simultaneous quantification of berberine and lysergol by HPLC-UV: evidence that lysergol enhances the oral bioavailability of berberine in rats. Biomed. Chromatogr. 26, 1170–1175 (2012).
Deng, Y. et al. Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 863, 195–205 (2008).
Zuo, F., Nakamura, N., Akao, T. & Hattori, M. Pharmacokinetics of berberine and its main metabolites in conventional and pseudo germ-free rats determined by liquid chromatography/ion trap mass spectrometry. Drug Metab. Dispos. 34, 2064–2072 (2006).
Pang, Y. N. et al. Transportation of berberine into HepG2, HeLa and SY5Y cells: a correlation to its anti-cancer effect. PLoS ONE 9, e112937 (2014).
Megyesi, M. & Biczók, L. Berberine alkaloid as a sensitive fluorescent probe for bile salt aggregates. J. Phys. Chem. B. 111, 5635–5639 (2007).
Wu, W., Yang, J., Du, L., Wu, H. & Li, C. Determination of ethambutol by a sensitive fluorescent probe. Spectrochim. Acta. A Mol. Biomol. Spectrosc. 79, 418–422 (2011).
Wu, F. et al. Simultaneous fluorescence light-up and selective multicolor nucleobase recognition based on sequence-dependent strong binding of berberine to DNA abasic site. Org. Biomol. Chem. 10, 3300–3307 (2012).
Schoonen, W. G. E. J., Stevenson, J. C. R., Westerink, W. M. A. & Horbach, G. J. Cytotoxic effects of 109 reference compounds on rat H4IIE and human HepG2 hepatocytes. III: Mechanistic assays on oxygen consumption with MitoXpress and NAD(P)H production with Alamar Blue™. Toxicol. In Vitro 26, 511–525 (2012).
Liu, J., He, C., Zhou, K., Wang, J. & Kang, J. X. Coptis extracts enhance the anticancer effect of estrogen receptor antagonists on human breast cancer cells. Biochem. Bioph. Res. Commun. 378, 174–178 (2009).
Tong, N. et al. Berberine sensitizes mutliple human cancer cells to the anticancer effects of doxorubicin in vitro. Oncol. Lett. 3, 1263–1267 (2012).
Chidambara Murthy, K. N., Jayaprakasha, G. K. & Patil, B. S. The natural alkaloid berberine targets multiple pathways to induce cell death in cultured human colon cancer cells. Eur. J. Pharmacol. 688, 14–21 (2012).
Wang, L. et al. Berberine induces caspase-independent cell death in colon tumor cells through activation of apoptosis-inducing factor. PLoS ONE 7, e36418 (2012).
Liu, B. et al. Berberine inhibits human hepatoma cell invasion without cytotoxicity in healthy hepatocytes. PLoS ONE 6, e21416 (2011).
Zhao, X. et al. Effect of berberine on hepatocyte proliferation, inducible nitric oxide synthase expression, cytochrome P450 2E1 and 1A2 activities in diethylnitrosamine- and phenobarbital-treated rats. Biomed. Pharmacother. 62, 567–572 (2008).
Guo, Y., Chen, Y., Tan, Z. R., Klaassen, C. D. & Zhou, H. H. Repeated administration of berberine inhibits cytochromes P450 in humans. Eur. J. Clin. Pharmacol. 68, 213–217 (2012).
Lee, A. S. Glucose-regulated proteins in cancer: molecular mechanisms and therapeutic potential. Nat. Rev. Cancer 14, 263–276 (2014).
Li, Y. et al. Bioactivities of berberine metabolites after transformation through CYP450 isoenzymes. J. Transl. Med. 9, 62 (2011).
Yu, Y. et al. Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mol. Cell 46, 7–17 (2012).
Wang, D. et al. The constitutive androstane receptor is a novel therapeutic target facilitating cyclophosphamide-based treatment of hematopoietic malignancies. Blood 121, 329–338 (2013).
Zelko, I., Sueyoshi, T., Kawamoto, T., Moore, R. & Negishi, M. The peptide near the C terminus regulates receptor CAR nuclear translocation induced by xenochemicals in mouse liver. Mol. Cell Biol. 21, 2838–2846 (2001).
Wang, H. et al. Human constitutive androstane receptor mediates induction of CYP2B6 gene expression by phenytoin. J. Biol. Chem. 279, 29295–29301 (2004).
Wu, L. et al. Altered methylation of IGF2 DMR0 is associated with neural tube defects. Mol. Cell Biochem. 380, 33–42 (2013).
Ehrich, M. et al. Quantitative high-throughput analysis of DNA methylation patterns by base-specific cleavage and mass spectrometry. Proc. Natl. Acad. Sci. 102, 15785–15790 (2005).
Acknowledgements
This study was supported by the National Natural Science Foundation of China (30973585, 91029747 and 81473276).
Author information
Authors and Affiliations
Contributions
L.Z., X.J.M., X.W., H.H.P., L.P., H.R. and L.Y. performed the experiments. H.B.W., Y.R.J. and L.Y. contributed plasmid/materials/analysis tools. All the authors analyzed the data. G.L.Z., L.Z. and L.Y. conceived and designed the experiments. G.L.Z., L.Z. and C.L. wrote the manuscript.
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Electronic supplementary material
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Zhang, L., Miao, XJ., Wang, X. et al. Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells. Sci Rep 6, 28116 (2016). https://doi.org/10.1038/srep28116
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep28116
This article is cited by
-
A Systematic Review of Drug Metabolism Studies of Plants With Anticancer Properties: Approaches Applied and Limitations
European Journal of Drug Metabolism and Pharmacokinetics (2020)
-
Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity
Chinese Medicine (2018)
-
Cell-specific pattern of berberine pleiotropic effects on different human cell lines
Scientific Reports (2018)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.