Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

RhoG regulates gene expression and the actin cytoskeleton in lymphocytes

Oncogene volume 22, pages 330–342 (2003)Cite this article

Abstract

RhoG, a member of the Rho family of GTPases, has been implicated as a regulator of the actin cytoskeleton. In this study, we show a novel function for the small GTPase RhoG on the regulation of the interferon-γ promoter and nuclear factor of activated T cells (NFAT) gene transcription in lymphocytes. Optimal function of RhoG for the expression of these genes requires a calcium signal, normally provided by the antigen receptor. In addition, RhoG potentiation of NFAT requires the indirect activity of Rac and Cdc42; however, pathways distinct from those activated by Rac and Cdc42 mediate RhoG activation of NFAT-dependent transcription. Using effector domain mutants of RhoG we found that its ability to potentiate NFAT-dependent transcription correlates with its capacity to increase actin polymerization, supporting the suggestion that NFAT-dependent transcription is an actin-dependent process. RhoG also promotes T-cell spreading on fibronectin, a property that is independent of its ability to enhance NFAT-dependent transcription. Hence, these results implicate RhoG in leukocyte trafficking and the control of gene expression induced in response to antigen encounter.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 6

Similar content being viewed by others

References

  • Angkachatchai V and Finkel TH . (1999). J. Immunol., 163, 3819–3825.

  • Arrieumerlou C, Randriamampita C, Bismuth G and Trautmann A . (2000). J. Immunol., 165, 3182–3189.

  • Benard V, Bohl BP and Bokoch GM . (1999). J. Biol. Chem., 274, 13198–13204.

  • Billadeau DD, Brumbaugh KM, Dick CJ, Schoon RA, Bustelo XR and Leibson PJ . (1998). J. Exp. Med., 188, 549–559.

  • Bishop AB and Hall A . (2000). Biochem. J., 348, 241–255.

  • Blangy A, Vignal E, Schmidt S, Debant A, Gauthier-Rouviere C, and Fort P . (2000). J. Cell. Sci., 113, 729–739.

  • Bustelo XR . (2000). Mol. Cell. Biol., 20, 1461–1477.

  • Corre I, Gomez M, Vielkind S and Cantrell DA . (2001). J. Exp. Med., 194, 903–913.

  • Costello PS, Cleverley SC, Galandrini R, Henning SW and Cantrell DA . (2000). J. Exp. Med., 192, 77–85.

  • Crabtree GR . (1989). Science, 243, 355–361.

  • Croker BA, Tarlinton DM, Cluse LA, Tuxen AJ, Light A, Yang FC, Williams DA and Roberts AW . (2002). J. Immunol., 168, 3376–3386.

  • D'Souza-Schorey C, Boettner B and Van Aelst L . (1998). Mol. Cell. Biol., 17, 3936–3946.

  • Estrach S, Schmidt S, Diriong S, Penna A, Blangy A, Fort P and Debant A . (2002). Curr. Biol., 12, 307–312.

  • Faull RJ, Kovach NL, Harlan JM and Ginsberg MH . (1994). J. Exp. Med., 179, 1307–1316.

  • Gabig TG, Crean CD, Mantel PL and Rosli R . (1995). Blood, 85, 804–811.

  • Galandrini R, Henning SW and Cantrell DA . (1997). Immunity, 7, 163–174.

  • Gauthier-Rouvire C, Vignal E, Mèriane M, Roux P, Montcourier P and Fort P . (1998). Mol. Biol. Cell., 9, 1379–1394.

  • Genot E, Cleverley S, Henning S and Cantrell D . (1996). EMBO. J., 15, 3923–3933.

  • Gomez M, Kioussis D and Cantrell DA . (2001). Immunity, 15, 703–713.

  • Gomez M, Tybulewicz V and Cantrell DA . (2000). Na. Immunol., 1, 348–352.

  • Hashimoto A, Okada H, Jiang A, Kurosaki M, Greenberg S, Clark EA and Kurosaki T . (1998). J. Exp. Med., 188, 1287–1295.

  • Henning SW and Cantrell DA . (1998). J. Exp. Med., 188, 931–939.

  • Henning SW, Galandrini R, Hall A and Cantrell DA . (1997). EMBO. J., 16, 2397–2407.

  • Holsinger LJ, Graef IA, Swat W, Chi T, Bautista DM, Davidson L, Lewis RS, Alt FW and Crabtree GR . (1998). Curr. Biol., 8, 563–572.

  • Jacinto E, Werlen G and Karin M . (1998). Immunity, 8, 31–41.

  • Katoh H, Yasui H, Yamaguchi Y, Aoki J, Fujita H, Mori K and Negishi M . (2000). Mol. Cell. Biol., 20, 7378–7387.

  • Kitayama H, Matsuzaki T, Ikawa Y and Noda M . (1990). Proc. Natl. Acad. Sci. USA, 87, 4284–4288.

  • Krawczyk C, Bachmaier K, Sasaki T, Jones RG, Snapper SB, Bouchard D, Kozieradzki I, Ohashi PS, Alt FW and Penninger JM . (2000). Immunity, 13, 463–473.

  • Kuhne MR, Ku G and Weiss A . (2000). J. Biol. Chem., 275, 2185–2190.

  • Lamarche N, Tapon N, Stowers L, Burbelo PD, Aspenstrom P, Bridges T, Chant J and Hall A . (1996). Cell, 87, 519–529.

  • Lang P, Guizani L, Vitte_Mony I, Stancou R, Dorseuil O, Gacon G and Bertoglio J . (1992). J. Biol. Chem., 267, 11677–11680.

  • Li B, Yu H, Zheng W, Voll R, Na S, Roberts AW, Williams DA, Davis RJ, Ghosh S and Flavell RA . (2000). Science, 288, 2219–2222.

  • Lores P, Morin L, Luna R and Gacon G . (1997). Oncogene, 15, 601–605.

  • Maly FE, Quilliam LA, Dorseuil O, Der CJ and Bokoch GM . (1994). J. Biol. Chem., 269, 18743–18746.

  • Manser E, Loo TH, Koh CG, Zhao ZS, Chen XQ, Tan L, Tan I, Leung T and Lim L . (1998). Mol. Cell, 1, 183–192.

  • Movilla N and Bustelo XR . (1999). Mol. Cell. Biol., 19, 7870–7885.

  • Na S, Li B, Grewal IS, Enslen H, Davis RJ, Hanke JH and Flavell RA . (1999). Oncogene, 18, 7966–7974.

  • Reynolds LF, Smyth L, Norton T, Freshney N, Downward J, Kioussis D and Tybulewicz VLJ . (2002). J Exp Med., 195, 1103–1114.

  • Rincon M, Enslen H, Raingeaud J, Recht M, Zapton T, Su MSS, Penix LA, Davis RJ and Flavell RA . (1998). EMBO J., 17, 2817–2829.

  • Roux P, Gauthier-Rouvire C, Doucet-Brutin S and Fort P . (1997). Curr. Biol., 7, 629–637.

  • Schuebel KE, Bustelo XR, Nielsen DA, Song BJ, Barbacid M, Goldman D and Lee IJ . (1996). Oncogene, 13, 363–371.

  • Schuebel KE, Movilla N, Rosa JL and Bustelo XR . (1998). EMBO J., 17, 6608–6621.

  • Stowers L, Yelon D, Berg LJ and Chant J . (1995). Proc. Natl. Acad. Sci. USA, 92, 5027–5031.

  • Tapon N, Nagata K, Lamarche N and Hall A . (1998). EMBO J., 17, 1395–1404.

  • vandenBerghe N, Cool RH, Horn G and Wittinghofer A . (1997). Oncogene, 15, 845–850.

  • Vignal E, Blangy A, Martin M, Gauthier-Rouviere C and Fort P . (2001). Mol. Cell. Biol., 21, 8022–8034.

  • Villalba M, Coudronniere N, Deckert M, Teixeiro E, Mas P and Altmann A . (2000). Immunity, 12, 151–160.

  • Vincent S, Jeanteur P and Fort P . (1992). Mol. Cell. Biol., 12, 3138–3148.

  • Wardenburg JB, Pappu R, Bu J-Y, Meyer B, Chernoff J, Straus D and Chan AC . (1998). Immunity, 9, 607–616.

  • Westwick JK, Lambert QT, Clark GJ, Symons M, Van Aelst L, Pestell RG and Der CJ . (1997). Mol. Cell. Biol., 17, 1324–1335.

  • Wielowieyski PA, Sevinc S, Guzzo R, Salih M, Wigle JT and Tuana BS . (2000). J. Biol. Chem., 275, 38474–38481.

  • Yablonski D, Kane LP, Qian D and Weiss A . (1998). EMBO J., 17, 5647–5657.

Download references

Acknowledgements

We thank Nick Kistakis and Maria Manifava for advice and assistance with microscopy, Sue Robinson for secretarial assistance and Babraham Institute Sequencing Support for DNA analysis. This work was funded in part by a Biotechnology and Biological Sciences Research Council Competitive Strategic Grant and by grants from the Leukaemia Research Fund and Cancer Research Campaign (MT).

Author information

Author notes

  1. Gina Doody

    Present address: Centre de Recherches en Biochimie Macromoleculaire, CNRS IFR24-UPR 1086, 1919 route de Mende, Montpellier, 34293, Cedex 5, France

  2. Phillipe Fort

    Present address: Molecular Medicine Unit, University of Leeds, St James's University Hospital, Leeds, LS9 7TF, UK

Authors and Affiliations

  1. Laboratory for Lymphocyte Signalling and Development, Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge, CB2 4AT, UK

    Elena Vigorito, Simon McAdam, Gina Doody & Martin Turner

  2. Department of Immunology, Mayo Graduate Schools, Mayo Clinic, Rochester, 55905, MN, USA

    Daniel D Billadeu & Doris Savoy

Authors
  1. Elena Vigorito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daniel D Billadeu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Doris Savoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Simon McAdam
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gina Doody
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Phillipe Fort
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Martin Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elena Vigorito.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vigorito, E., Billadeu, D., Savoy, D. et al. RhoG regulates gene expression and the actin cytoskeleton in lymphocytes. Oncogene 22, 330–342 (2003). https://doi.org/10.1038/sj.onc.1206116

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206116

Keywords

This article is cited by

Search

Advanced search

Quick links