Abstract
The phosphotyrosine (pTyr) adapter Shc/ShcA is a major connector in various tyrosine kinase signalings following a variety of stimulation such as growth factor/neurotrophin, as well as in those following calcium influx and integrin activation. As in other tissues, Shc has been implicated in neuronal signalings; however, recent evidence suggests that N-Shc/ShcC and Sck/ShcB would take over most of the roles of Shc in mature central neurons, and switching phenomena between Shc and N-Shc expression were observed in several neuronal paradigms. Little is, however, known as to the signal-output differences between Shc and N-Shc. Here we determined the efficacy of Shc and N-Shc toward Erk activation in NGF-treated PC12 cells, and found that N-Shc transduced Grb2/Sos/Ras-dependent Erk activation less efficiently than Shc. This was mainly because N-Shc has only one high-affinity Grb2-binding site, whereas Shc has two such sites. Phosphopeptide mapping revealed that N-Shc has novel tyrosine-phosphorylation sites at Y259/Y260 and Y286; in vivo-phosphorylation of these tyrosines was demonstrated by site-specific anti-pTyr antibodies. Phosphorylated Y286 bound to several proteins, of which one was Crk. The pY221/pY222 site, corresponding to one of the Grb2-binding sites of Shc, also preferentially bound to Crk. The phosphorylation-dependent interaction between N-Shc and Crk was demonstrated in vitro and in vivo. These results indicate that N-Shc has specific features of signal-output, and further suggest that the switching between Shc and N-Shc during neural development and regeneration would lead to differentiation of downstream signalings.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Anderson S, Davis DN, Dahlbäck H, Jörnvail H, Russel DW . 1989 J. Biol. Chem. 264: 8222–8229
Bonfini L, Migliaccio E, Pelicci G, Lanfrancone L, Pelicci PG . 1996 Trends Biol. Sci. 21: 257–261
Bourne HR . 1995 Nature 376: 727–729
Boyle WJ, van der Geer P, Hunter T . 1991 Methods Enzymol. 201: 110–149
Busca R, Abbe P, Mantoux F, Aberdam E, Peyssonnaux C, Eychene A, Ortonne JP, Ballotti R . 2000 EMBO J. 19: 2900–2910
Conti L, De Fraja C, Gulisano M, Migiaccio E, Govoni S, Cattaneo E . 1997 Proc. Natl. Acad. Sci. USA 94: 8185–8190
El-Shemerly MYM, Besser D, Nagasawa M, Nagamine Y . 1997 J. Biol. Chem. 272: 30599–30602
Escalante M, Courtney J, Chin WG, Teng KK, Kim JI, Fajardo JE, Mayer BJ, Hempstead BL, Birge RB . 2000 J. Biol. Chem. 275: 24787–24797
Gotoh N, Tojo A, Shibuya M . 1996 EMBO J. 15: 6197–6204
Gotoh N, Toyoda M, Shibuya M . 1997 Mol. Cell. Biol. 17: 1824–1831
Harmer SL, DeFranco AL . 1997 Mol. Cell. Biol. 17: 4087–4095
Hashimoto A, Kurosaki M, Gotoh N, Shibuya M, Kurosaki T . 1999 J. Biol. Chem. 274: 20139–20143
Hashimoto Y, Matsuoka K, Takenawa T, Muroya K, Hattori S, Nakamura S . 1994 Oncogene 9: 869–875
Hauser CA, Westwick JK, Quilliam LA . 1995 Methods Enzymol. 255: 395–412
Howe LR, Leevers SJ, Gomez N, Nakielny S, Cohen P, Marshall CJ . 1992 Cell 16: 335–342
Ichiba T, Kuraishi Y, Sakai O, Nagata S, Groffen J, Kurata T, Hattori S, Matsuda M . 1997 J. Biol. Chem. 272: 22215–22220
Iwasaki Y, Nishiyama H, Suzuki K, Koizumi S . 1997 Biochemistry 36: 2694–2700
Kao S, Jaiswal RK, Kolch W, Landreth GE . 2001 J. Biol. Chem. 276: 18169–18177
Kojima T, Yoshikawa Y, Takada S, Sato M, Nakamura T, Takahashi N, Copeland NG, Gilbert DJ, Jenkins NA, Mori N . 2001 Biochem. Biophys. Res. Commun. 284: 1039–1047
Lai KV, Pawson T . 2000 Genes Dev. 14: 1132–1145
Luschnig S, Krauss J, Bohmann K, Desjeux I, Nüsslein-Volhard C . 2000 Mol. Cell 5: 231–241
Matsuda M, Hashimoto Y, Muroya K, Hasegawa H, Kurata T, Tanaka S, Nakamura S, Hattori S . 1994 Mol. Cell. Biol. 14: 5495–5500
Matsuda M, Kurata T . 1996 Cell Signal 8: 335–340
Migliaccio E, Giorgio M, Mele S, Pelicci G, Reboldi P, Pandolfi PP, Lanfrancone L, Pelicci PG . 1999 Nature 402: 309–313
Miki H, Miura K, Matsuoka K, Nakata T, Hirokawa N, Orita S, Kaibuchi K, Takai Y, Takenawa T . 1994 J. Biol. Chem. 269: 5489–5492
Minichiello L, Casagranda F, Tatche RS, Stucky CL, Postigo A, Lewin GR, Davis AM, Klein R . 1998 Neuron 21: 335–345
Nakamura T, Muraoka S, Sanokawa R, Mori N . 1998 J. Biol. Chem. 273: 6960–6967
Nakamura T, Sanokawa R, Sasaki Y, Ayusawa D, Oishi M, Mori N . 1996 Oncogene 13: 1111–1121
Nakashima N, Rose DW, Xiao S, Egawa K, Martin SS, Haruta T, Saltiel AR, Olefsky JM . 1999 J. Biol. Chem. 274: 3001–3008
O'Bryan JP, Martin CB, Songyang Z, Cantley LC, Der CJ . 1996a J. Biol. Chem. 271: 11787–11791
O'Bryan JP, Songyang Z, Cantley LC, Der CJ, Pawson T . 1996b Proc. Natl. Acad. Sci. USA 93: 2729–2734
Obermeier A, Bradshaw RA, Seedorf K, Choidas A, Schlessinger J, Ullrich A . 1994 EMBO J. 13: 1585–1590
Okabayashi Y, Sugimoto Y, Totty NF, Hsuan J, Kido Y, Sakaguchi K, Gout I, Waterfield MD, Kasuga M . 1996 J. Biol. Chem. 271: 5265–5269
Pawson T, Saxton TM . 1999 Cell 97: 675–678
Pelicci G, Dente L, Giuseppe AD, Verducci-Galletti B, Giuli S, Mele S, Vetriani C, Giorgio M, Pandolfi PP, Cesareni G, Pelicci PG . 1996 Oncogene 13: 633–641
Ribon V, Saltiel AR . 1996 J. Biol. Chem. 271: 7375–7380
Rozakis-Adcock M, McGlade J, Mbamalu G, Pelicci G, Daly R, Li W, Batzer A, Thomas S, Brugge J, Pelicci PG, Schlessinger J, Pawson T . 1992 Nature 360: 689–692
Sakai R, Henderson JT, O'Bryan JP, Elia AJ, Saxton TM, Pawson T . 2000 Neuron 28: 819–833
Shields JM, Pruitt K, McFall A, Shaub A, Der CJ . 2000 Trends Cell Biol. 10: 147–154
Simcox ME, Huvar A, Simcox TG, Vega Q . 1994 Strategies 7: 68–69
Stephens RM, Loeb DM, Copeland TD, Pawson T, Greene LA, Kaplan DR . 1994 Neuron 12: 691–705
Tanabe K, Kiryu-Seo S, Nakamura T, Mori N, Tsujino H, Ochi T, Kiyama H . 1998 Brain Res. Mol. Brain Res. 53: 291–296
Tanaka S, Ouchi T, Hanafusa H . 1997 Proc. Natl. Acad. Sci. USA 94: 2356–2361
Thomas D, Bradshaw RA . 1997 J. Biol. Chem. 272: 22293–22299
Torres M, Bogenmann E . 1996 Oncogene 12: 77–86
van der Geer P, Wiley S, Gish GD, Lai VK, Stephens R, White MF, Kaplan D, Pawson T . 1996a Proc. Natl. Acad. Sci. USA 93: 963–968
van der Geer P, Wiley S, Gish GD, Pawson T . 1996b Curr. Biol. 6: 1435–1444
Wary KK, Mariotti A, Zurzolo C, Giancotti FG . 1998 Cell 94: 625–634
Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ . 1995 Science 269: 403–407
Yamashita S, Mochizuki N, Ohba Y, Tobiume M, Okada Y, Sawa H, Nagashima K, Matsuda M . 2000 J. Biol. Chem. 275: 25488–25493
York RD, Yao H, Dillon T, Ellig CL, Eckert SP, McCleskey EW, Stork PJ . 1998 Nature 392: 622–626
Zwartkruis FJ, Wolthuis RM, Nabben NM, Franke B, Bos JL . 1998 EMBO J. 17: 5905–5912
Acknowledgements
We thank Akiko Nakai, Sachiyo Mimaki, Itsuko Nakano and Yumiko Kadokawa for technical assistance, Chris Marshall and Michiyuki Matsuda for providing EXV3ERK2–Myc and pCAGGS–CrkI, respectively and Hiroshi Morii and Masahiro Sato for comments on the manuscript. This work was supported by a grant from CREST, JST and also, in part, by Strategic Promotion System for Brain Science from STA and Funds for Comprehensive Research on Aging and Health from the Ministry of Health, Labor and Welfare to N Mori.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nakamura, T., Komiya, M., Gotoh, N. et al. Discrimination between phosphotyrosine-mediated signaling properties of conventional and neuronal Shc adapter molecules. Oncogene 21, 22–31 (2002). https://doi.org/10.1038/sj.onc.1205019
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1205019
Keywords
This article is cited by
-
Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells
Scientific Reports (2020)
-
Distinct role of ShcC docking protein in the differentiation of neuroblastoma
Oncogene (2009)
-
RAI(ShcC/N-Shc)-dependent recruitment of GAB1 to RET oncoproteins potentiates PI3-K signalling in thyroid tumors
Oncogene (2005)