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The rapid destabilization of p53 mRNA in immortal chicken embryo fibroblast cells

Oncogene volume 20, pages 5118–5123 (2001)Cite this article

Abstract

The steady-state levels of p53 mRNA were dramatically lower in immortal chicken embryo fibroblast (CEF) cell lines compared to primary CEF cells. In the presence of cycloheximide (CHX), the steady-state levels of p53 mRNA markedly increased in immortal CEF cell lines, similar to levels found in primary cells. The de novo synthetic rates of p53 mRNA were relatively similar in primary and immortal cells grown in the presence or absence of CHX. Destabilization of p53 mRNA was observed in the nuclei of immortal, but not primary, CEF cells. The half-life of p53 mRNA in primary cells was found to be a relatively long 23 h compared to only 3 h in immortal cells. The expression of transfected p53 cDNA was inhibited in immortal cells, but restored upon CHX treatment. The 5′-region of the p53 mRNA was shown to be involved in the rapid p53 mRNA destabilization in immortal cells by expression analysis of 5′- and 3′-deleted p53 cDNAs as well as fusion mRNA constructs of N-terminal p53 and N-terminal deleted LacZ genes. Together, it is suggestive that the downregulation of p53 mRNA in immortal CEF cells occurs through a post-transcriptional destabilizing mechanism.

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Abbreviations

HPV:

human papillomavirus

SV40:

simian virus 40

CEF:

chicken embryonic fibroblast

RT–PCR:

reverse transcriptase-PCR

ActD:

actinomycin D

ev-0:

endogenous virus-free

SPAFAS:

specific pathogen free avian supply

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

CHX:

cycloheximide

CMV:

cytomegalovirus

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Acknowledgements

This work was supported, in part, by USDA/NRICGP grant #9603280 and a grant from American Home Products (Fort Dodge Animal Health) to DN Foster.

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Authors and Affiliations

  1. Department of Animal Science, University of Minnesota, St Paul, Minnesota, MN 55108, USA

    Hyunggee Kim, Linda K Foster, James Farris & Douglas N Foster

  2. School of Agricultural Biotechnology, Seoul National University, Suwon, 441-744, Korea

    Seungkwon You

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  1. Hyunggee Kim
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Correspondence to Douglas N Foster.

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Kim, H., You, S., Foster, L. et al. The rapid destabilization of p53 mRNA in immortal chicken embryo fibroblast cells. Oncogene 20, 5118–5123 (2001). https://doi.org/10.1038/sj.onc.1204664

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