Abstract
Apoptosis, the process whereby cells activate an intrinsic death program, can be induced in HeLa cells by TNF-α treatment. The aims of the present study were (i) to examine the precise role and the origin of Reactive Oxygen Species (ROS) in the TNF-α-induced programmed cell death, (ii) to characterize and order the morphological and mitochondrial changes associated with this process and (iii) to link these events with the activation of caspases. Analyses were performed on TNF-α-treated cells in the presence of an anti-oxidant, or of a general caspase inhibitor. To assess the role of mitochondria in the cell death signal transduction, these studies were also realized on HeLa-variant cell lines lacking functional mitochondrial respiratory chain. We show that at least two separate signaling cascades, both mediated by Z-VAD-sensitive caspase(s), contribute to the TNF-α-induced apoptosis of HeLa cells. One signaling pathway involves an early mitochondria-dependent ROS production, the other being ROS-independent.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fraisse, Cd., Rincheval, V., Risler, Y. et al. TNF-α activates at least two apoptotic signaling cascades. Oncogene 17, 1639–1651 (1998). https://doi.org/10.1038/sj.onc.1202094
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1202094
Keywords
This article is cited by
-
Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands
Scientific Reports (2024)
-
Computational modelling of TNFα related pathways regulated by neuroinflammation, oxidative stress and insulin resistance in neurodegeneration
Applied Network Science (2020)
-
Anticancer and cytotoxic effects of troxerutin on HeLa cell line: an in-vitro model of cervical cancer
Molecular Biology Reports (2020)
-
Neuroprotective Effects of Chrysin on Diclofenac-Induced Apoptosis in SH-SY5Y Cells
Neurochemical Research (2020)
-
Differential effects of Bcl-2 and caspases on mitochondrial permeabilization during endogenous or exogenous reactive oxygen species-induced cell death
Cell Biology and Toxicology (2012)