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Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects

International Journal of Obesity volume 24, pages 70–74 (2000)Cite this article

Abstract

OBJECTIVE: To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans.

DESIGN: Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.±1.9 kg/m2, aged 48±3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9±0.1 MJ/day).

MEASUREMENTS: Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR.

RESULTS: VLCD induced weight loss (5.8±0.8 kg) and decreased plasma PAI-1 concentration (−26% (P<0.01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively.

CONCLUSION: These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects.

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Acknowledgements

The authors thank Drs MC Alessi and N Ventura for the gift of human PAI-1 cDNA and for their help in the elaboration of PAI-1 mRNA assay. This work was supported by grants from Le Ministère de la Santé (Programme Hospitalier de Recherche Clinique no. AOA94042).

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Authors and Affiliations

  1. Service de Biochimie, Hôpital de la Salpêtrière, AP-HP, Paris

    J-P Bastard, C Jardel, P Blondy & B Hainque

  2. INSERM U-449, Faculté de Médecine RTH Laënnec, Lyon

    H Vidal & P Vallier

  3. Service d'Endocrinologie, Hôpital de la Pitié, AP-HP, Paris

    E Bruckert & G Turpin

  4. Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, CNRS, Meudon

    D Robin & C Forest

  5. Institut Fédératif de Recherche Coeur, Muscle et Vaisseaux, Groupe Hospitalier Pitié-Salpêtrière, Paris, AP-HP, France

    J-P Bastard, C Jardel, E Bruckert, G Turpin & B Hainque

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  1. J-P Bastard
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  2. H Vidal
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  10. B Hainque
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Correspondence to J-P Bastard.

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Bastard, JP., Vidal, H., Jardel, C. et al. Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects. Int J Obes 24, 70–74 (2000). https://doi.org/10.1038/sj.ijo.0801087

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