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Preterm infant formula supplementation with α linolenic acid and docosahexaenoic acid

European Journal of Clinical Nutrition volume 57, pages 727–734 (2003)Cite this article

Abstract

Objectives: To investigate if supplementation of preterm infant formula with a high docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) ratio together with α-linolenic acid (ALA) was able to maintain plasma and red blood cell DHA levels similar to that obtained with breast milk feeding without altering n-6 fatty acid status.

Design and subjects: Preterm infants of mothers who elected not to breast feed (n=13) were assigned to ALA- and DHA-enriched formula (DHA group: DHA/EPA=5/l). Infants fed breast milk (n=25) constituted a reference group (BM group). Anthropometric and fatty acid parameters (plasma phospholipids, cholesterol esters, triglycerides and red blood cell phosphatidylethanolamine, PL, CE, TG, RBC-PE, respectively) were obtained after 2 days (D2) and 15 days (D15) of enteral feeding and at the 37th week (W37) of post-conception age and 1 month later (W37+30) in the DHA group. Mean DHA intake ranged between 16.5±1.6 and 17.9±2.9 mg/kg/day between D2 and W37+30.

Results: At W37, infant weights, heights, and head circumferences were similar in DHA and BM groups. PL DHA was maintained in the DHA group at the same level as in the BM group and the same for DHA in PE at W37. In RBC-PE and at W37, AA status was the same in both groups. In PL, AA levels remained very stable throughout the study; however, in the DHA group AA levels in PL remained in the range observed with standard formulas.

Conclusion: The combined 18:3 n-3 and DHA supplementation of infant formula with DHA/EPA ratio 5/l is compatible with growth and n-3 fatty acid metabolism similar to that of preterm infants fed human milk.

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References

  • Babin F, Rodriguez A, Vandeputte B, Mendy F & Descomps B (2000): Alpha linolenic acid in cholesterol esters: a marker of alphalinolenic acid intake in newborns. Eur. J. Clin. Nutr. 54, 840–843.

    Article  CAS  Google Scholar 

  • Balcao WH, Kemppinen A, Malcata FX & Kalo PJ (1998): Modification of butterfat by selective hydrolysis and esterification by lipase: process and product characterization. J. Am. Oil Chem. Soc. 75, 1347–1358.

    Article  CAS  Google Scholar 

  • Billeaud C, Bougle D, Sarda P, Combe N, Mazette S, Babin F, Entressangles B, Descomps B, Nouvelot A & Mendy F (1997): Effects of preterm infant formula supplementation with α-linolenic acid with a linoleate/α-linolenate ratio of 6/1: a multicentric study. Eur. J. Clin. Nutr. 51, 520–526.

    Article  CAS  Google Scholar 

  • Birch EE, Birch DG, Hoffman DR & Uauy R (1992): Dietary essential fatty acid supply and visual acuity development. Invest. Opthalmol. Vis. Sci. 33, 3242–3253.

    CAS  Google Scholar 

  • Birch EE, Garfield S, Hoffman DR, Uauy R & Birch DG (2000): A randomized controlled trial of early dietary supply of long-chain polyunsaturated fatty acids and mental development in term infants. Dev. Med. Child. Neurol. 42, 174–181.

    Article  CAS  Google Scholar 

  • Bourre JM, François M, Youyou A, Dumont O, Piciotti M & Pascal G (1989): The effects of dietary alpha-linolenic acid on the composition of nerve membranes, enzymatic activity, amplitude of electrophysiological parameters, resistance to poisons and performance of learning tasks in rats. J. Nutr. 119, 1880–1892.

    Article  CAS  Google Scholar 

  • Carlson SE, Cooke PJ, Werkman SH & Tolley EA (1992): First year growth of preterm infants fed standard compared to marine oil n-3 supplemented formula. Lipids 27, 901–907.

    Article  CAS  Google Scholar 

  • Carlson SE, Werkman SH, Rhodes PG & Tolley EA (1993): Visual acuity development in healthy preterm infants: effect of marine oil supplementation. Am. J. Clin. Nutr. 58, 35–42.

    Article  CAS  Google Scholar 

  • Carnielli VP, Pederzini F, Vittorangeli R, Lujendjik IHT, Pedrotti D & Sauer PJJ (1996): Plasma and red blood cell fatty acid of very low birth weight infants fed exclusively with expressed preterm human milk. Pediatr. Res. 39, 671–679.

    Article  CAS  Google Scholar 

  • Clandinin MT, Chappell JE, Heim T, Swyer PR & Chance GW (1981): Fatty acids utilization in perinatal de novo synthesis of tissues. Early Hum. Dev. 5, 355–366.

    Article  CAS  Google Scholar 

  • Clandinin MT, Chappell JE, Leong S, Heim T, Swyer PR & Chance GW (1980): Intrauterine fatty acid accretion rates in human brain: implication for fatty acid requirements. Early Hum. Dev. 4, 121–129.

    Article  CAS  Google Scholar 

  • Clandinin MT, Van Aerde JE, Parrott A, Field CJ, Euler AR & Lien E (1999): Assessment of feeding different amounts of arachidonic and docosahexaenoic acids in pretetrm infant formulas on the fatty acid. Acta Paediatr. 88, 890–896.

    Article  CAS  Google Scholar 

  • Demmelmair H, Feldt F, Horwath I, Niederland T, Ruzinko V, Raederstorff D, De Min C, Muggli R & Kolezko B (2001): Influence of formulas with borage oil or borage oil plus fish oil on the arachidonic acid status in premature infants. Lipids 36, 555–566.

    Article  CAS  Google Scholar 

  • Folch J, Lees M & Sloane Stanley GH (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509.

    CAS  Google Scholar 

  • Gibson RA, Makrides M, Neumann MA, Simmer K, Mantzioris E & James MJ (1994): Ratios of linoleic acid to α-linoleic acid in formulas for term infants. J. Pediatr. 125, S48–S55.

    Article  CAS  Google Scholar 

  • Hornstra G (2000): Essential fatty acids in mothers and their neonates. Am. J. Clin. Nutr. 7, 1262S–1269S.

    Article  Google Scholar 

  • Lapillonne A, Picaud JC, Chirouze V, Goudable J, Reygrobellet B, Claris O & Salle BL (2000): The use of low-EPA fish oil for long-chain polyunsaturated fatty acid supplementation of preterm infants. Pediatr. Res. 48, 835–841.

    Article  CAS  Google Scholar 

  • Makrides M, Neumann MA, Byard RW, Simmer K & Gibson RA (1994): Fatty acid composition of brain retina, and erythrocytes in breast- and formula-fed infants. Am. J.Clin. Nutr. 60, 189–194.

    Article  CAS  Google Scholar 

  • Martinez M (1992): Tissue levels of polyunsaturated fatty acids during early human development. J. Pediatr. 120, S129–S138.

    Article  CAS  Google Scholar 

  • Rapoport SI, Chang MC & Spector AA (2001): Delivery and turnover of plasma-derived essential PUFAs in mammalian brain. J. Lipid. Res. 42, 678–685.

    CAS  PubMed  Google Scholar 

  • Reisbick S, Neuringer M & Connor WE (1996): Effects of n-3 fatty acid deficiency in non human primates. In Recent Developments in Infant Nutrition, eds JO Bindels, AC Goedhart & HKA Visser, Dordrecht: Kluwer. pp 157–172.

    Chapter  Google Scholar 

  • Rietsh J (1982): Etude de la structure glycéridique de la nouvelle huile de Colza. Rev. Frs. Corps Gras. 29, 75–77.

    Google Scholar 

  • Rodriguez A, Sarda P, Nessmann C, Boulot P, Leger CL & Descomps B (1998a): Δ6- and Δ5-desaturase activity in human foetal liver: kinetic aspects. J. Lipid Res. 39, 1825–1832.

    CAS  PubMed  Google Scholar 

  • Rodriguez A, Sarda P, Nessmann C, Boulot P, Poisson JP, Leger CL & Descomps B (1998b): Fatty acid desaturase activities and long chain polyunsaturated fatty acid composition in human liver between the 17th and 36th gestation week. Am. J. Obstet. Gynecol. 179, 1063–1070.

    Article  CAS  Google Scholar 

  • Salem N, Wegher B, Mena P & Uauy R (1996): Arachidonic and docosahexaenoic acids are biosynthesized from their 18-carbon precursors in human infants. Proc. Natl Acad. Sci. 93, 49–54.

    Article  CAS  Google Scholar 

  • Uauy RD, Birch DG, Birch EE, Tysion JE & Hoffman DR (1990): Effect of dietary omega 3 fatty acids on retinal function of very-low birth-weight neonates. Pediatr. Res. 8, 485–492.

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biochimie A, EA Nutrition Humaine et Athérogenèse, UMI, Institut de Biologie, Montpellier, France

    A Rodriguez, C Lauret & B Descomps

  2. CETIOM, Paris, France

    F Mendy

  3. Service de Pédiatrie II, CHU de Montpellier, France

    P Sarda

  4. Roche Vitamins SA, Human Nutrition Research, Bâle, France

    D Raederstorff

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  1. A Rodriguez
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  2. D Raederstorff
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  6. B Descomps
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Correspondence to B Descomps.

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Rodriguez, A., Raederstorff, D., Sarda, P. et al. Preterm infant formula supplementation with α linolenic acid and docosahexaenoic acid. Eur J Clin Nutr 57, 727–734 (2003). https://doi.org/10.1038/sj.ejcn.1601604

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