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Post-Transplant Events

Plasma antioxidants in subjects before hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 38, pages 513–520 (2006)Cite this article

Abstract

Chemo-irradiation induced oxidative damage to vascular endothelium may contribute to pulmonary complications of hematopoietic stem cell transplantation (HSCT). We measured antioxidants, markers of oxidative stress and plasma antioxidant capacity in plasma or serum from 24 subjects at day 7 before HSCT and 20 control subjects. The plasma concentration of extracellular glutathione peroxidase (GPX-3) was significantly reduced in the HSCT subjects compared with controls (HSCT: 98±42 μg/ml, control: 169±56 μg/ml, P<0.0001). The concentration of γ-tocopherol was significantly higher in the HSCT subjects compared with controls (HSCT: 207±103 μg/dl; Control: 98±52 μg/dl; P=0.0002). The plasma concentrations of protein carbonyl, nitrotyrosine, malondialdehyde, α-tocopherol, vitamin A, homocysteine, cysteine and cysteinylglycine did not differ between HSCT and control subjects. Plasma from HSCT subjects was as effective as control plasma in quenching menadione-induced intracellular reactive oxygen species production in human microvascular endothelial cells. In summary, subjects before HSCT have significantly reduced plasma concentrations of GPX-3, elevated plasma γ-tocopherol yet retains the ability to quench an acute oxidative stress. These changes may play a role in chronic oxidative stress in the HSCT population.

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Acknowledgements

We thank all the subjects who enrolled in the study, the staff of the BMT unit at Tufts-New England Medical Center and the T-NEMC IRB for their help in getting this project completed.

This work was supported by: NIH K23HL04411 and USDA ARS Cooperative Agreement No 58-1950-4-401.

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Authors and Affiliations

  1. Critical Care and Sleep Division, Department of Medicine, Pulmonary, Tufts-New England Medical Center, Tupper Research Institute, Tufts University School of Medicine, Boston, MA, USA

    A C White, A M Sousa, B L Fanburg & U S Kayyali

  2. Division of Hematology/Oncology, Department of Medicine, Tufts-New England Medical Center, Tupper Research Institute, Tufts University School of Medicine, Boston, MA, USA

    J Blumberg

  3. Jean Mayer USDA Human Nutrition Research Center on Aging, Boston, MA, USA

    H F Ryan

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  1. A C White
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Correspondence to A C White.

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White, A., Sousa, A., Blumberg, J. et al. Plasma antioxidants in subjects before hematopoietic stem cell transplantation. Bone Marrow Transplant 38, 513–520 (2006). https://doi.org/10.1038/sj.bmt.1705475

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