Correction to: Scientific Reports https://doi.org/10.1038/s41598-023-34659-x, published online 08 May 2023
The original version of this Article contained errors in Figures 2 and 4, where panels 2B and 4B respectively did not display correctly. The original Figures 2 and 4 and accompanying legends appear below.
Attenuation of mechanical stress induced-matrix metalloproteinase (MMP-13) expression by Hic-5 knockdown in human chondrocytes. (A) mRNA levels of Hic-5, MMPs, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in human chondrocytes exposed to mechanical stress (MS+) for 30 min or untreated (MS−). Cells were collected at 1 h after mechanical stress. (n = 8 biological replicates). (B) Changes in gene expression in response to Hic-5 knockdown in human chondrocytes with or without mechanical stress. Human chondrocytes were treated with Hic-5 siRNA (10 nM) or control siRNA (10 nM) for 24 h before stimulation by mechanical stress. (n = 4 biological replicates). Relative levels of mRNA were determined by quantitative reverse transcription-polymerase chain reaction. Values are the mean ± SEM. *P < 0.05; **P < 0.01; §P = 0.0572 by the unpaired t test in (A) or one-way analysis of variance with Tukey’s test for multiple comparisons in (B).
Upregulation of MMP-13 expression by nuclear Hic-5 in human chondrocytes. (A) Induction of MMP-13 in human chondrocytes exogenously expressing Hic-5 tagged with a nuclear localization signal (NLS-HA-Hic-5). Human chondrocytes were transfected with the NLS-HA-Hic-5 expression vector at the concentrations shown in the graph for 24 h. Hic-5 and MMP-13 expression was measured by quantitative polymerase chain reaction (n = 3 biological replicates). (B) Western blot of MMP-13 in human chondrocytes transfected with or without 0.2 µg of NLS-Hic-5 (n = 3 biological replicates). Values are the mean ± SEM. **P < 0.01 by the Kruskal–Wallis test, followed by Dunn’s multiple comparisons test in (A) or the unpaired t test in (B). Western blotting images were cropped, and full-length blots are included in Supplementary Fig. 1. (C) Double immunofluorescence staining of Hic-5 (green) and MMP-13 (red) in human chondrocytes transfected with or without NLS-HA-Hic-5. Nuclei were counterstained with DAPI (blue). Representative image was selected from 3 biological replicates. Original magnification: × 400. Bar = 50 μm.
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Miyauchi, A., Noguchi, M., Lei, X. et al. Author Correction: Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13. Sci Rep 13, 9589 (2023). https://doi.org/10.1038/s41598-023-36393-w
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DOI: https://doi.org/10.1038/s41598-023-36393-w
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