Abstract
Redox status influences the course of the inflammatory, metabolic, and proliferative liver diseases. Oxidative stress is thought to play a crucial and sustained role in the pathological progression of early steatosis to severe hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Oxidative stress induced by reactive oxygen species which are generated in the mitochondria can lead to chronic organelle damage in hepatocytes. Currently, the diagnosis of liver disease requires liver biopsy, which is invasive and associated with complications. The present report describes the development of a novel molecular probe, EDA-PROXYL, with higher reactivity and mitochondrial selectivity than standard carboxyl-PROXYL and carbamoyl-PROXYL probes. The membrane permeability of our probe improved in aqueous environments which led to increased accumulation in the liver and interaction of EDA-PROXYL with the carnitine transporter via the amine (NH3+) group further increased accumulation. This increased mitochondrial sensitivity and enhanced accumulation highlight the potential of EDA-PROXYL as a molecular probe for determining metabolic reactions of the mitochondria. Thus, this novel probe could be a tool for the evaluation of redox status of the mitochondria to assess the degree of liver injury and, ultimately, the response to pharmacological therapy.
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Introduction
Liver disease is the most predominant and significant cause of morbidity and mortality in the world and includes a range of conditions from early steatosis to severe hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma1. Liver disease can be influenced by risk factors such as obesity, alcohol, drugs, and exposure to other toxins2. As the liver is the main organ involved in detoxification and nutrient metabolism, it is more vulnerable to oxidative stress caused by the presence of toxic materials and metabolites in the body3. Therefore, oxidative stress plays an important role in the pathophysiology of various liver diseases.
Oxidative stress increases mitochondrial transcription and replication4. The electron transport chain is blocked in damaged mitochondria, resulting in the accumulation of reactive oxygen species (ROS)5 and increase in free fatty acid β-oxidation which promotes the accumulation of lipids. Increased ROS production initiates a self-sustaining loop that causes chronic organelle damage such as damage to mitochondrial DNA and iron-sulphur cluster enzymes6,7. Malondialdehyde and 4-hydroxynonenal are produced from lipid peroxidation and inhibit cytochrome c oxidase in mitochondrial complex IV of the hepatocytes8. In fact, various ultrastructural abnormalities and mitochondrial dysfunction are observed in hepatic disorders and some studies have revealed that redox status in the context of liver injury is related to the condition of the mitochondria as these are the main source of ROS9,10,11. Steatohepatitis is a type of liver disease that can progress to cirrhosis or end-stage liver disease, and may ultimately result in hepatocellular carcinoma12. Oxidative stress is thought to play a key role in the progression of steatohepatitis to fibrosis, cirrhosis, and liver cancer1. Currently, diagnosis of liver disease requires liver biopsy13,14 which has some drawbacks as it is an invasive procedure which may cause bleeding or infection and has been suggested to be prone to sampling errors and misdiagnosis15,16. Therefore, the development of a non-invasive technique is urgently required for specific diagnosis of hepatic disorders; the use of molecular probes is a promising approach.
In a previous report, we showed that early changes in mitochondrial redox metabolism can be detected using a redox-sensitive probe carbamoyl PROXYL (CmP) with in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI)17. The MRI signals from nuclei are increased by the use of DNP18 as this increases the electron paramagnetic resonance (EPR) frequency of free radicals prior to applying the pulse sequence of MRI, thus increasing the image intensity in tissues where free radicals are present. Nitroxyl radicals modulate the redox reaction and the rate of reduction relies upon the redox status which is influenced by factors such as the level of production of ROS and antioxidant activity. Some studies have demonstrated the utility of nitroxyl radicals to obtain information on redox status19,20,21, tissue partial pressure of oxygen22,23, pH24, and proteolytic activity25 through in vivo DNP-MRI in living mice.
The carboxyl-PROXYL (CxP) and CmP that were used as DNP contrast agents in the present study are blood-stable radical compounds used for different biophysical and biomedical experiments17,26,27. Previous studies have shown that CxP is membrane impermeable while CmP is membrane permeable28, which might influence the redox reaction of these agents with ROS. In addition, CmP may be reduced by mitochondrial redox metabolism in the inner membrane17,29. Furthermore, cytoplasmic components such as antioxidants and constituents of the electron transfer system of the mitochondrial membrane eliminate radicals from these probes30,31. Although the mitochondrial redox status was assessed using CmP17, a stronger DNP signal would enable more precise and fast three-dimensional (3D) imaging for assessment of hepatic steatosis. To overcome the current problems in the field, we designed and synthesized a novel molecular probe, EDA-PROXYL, using the basic skeleton of CmP with the aim of creating a probe with high sensitivity to the mitochondria and enhanced accumulation in the liver. This novel probe could represent a therapeutic approach for the precise evaluation of the redox state of the mitochondria in the context of liver disease.
Materials and methods
Chemicals
We purchased 3-Carboxy-2,2,5,5-tetramethyl-1-pyrrolidine-1-oxyl (Carboxy-PROXYL) and 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidine-1-oxyl (Carbamoyl-PROXYL) from Sigma-Aldrich (Saint Louis, MO, USA). Benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP) was purchased from Merck. Potassium cyanide (KCN) and potassium hexacyanoferrate (III) (K3[Fe(CN)]6) were obtained from Wako Pure Chemical Industries (Osaka, Japan). All other chemicals used in this study were reagent-grade quality and commercially available.
Synthesis of EDA-PROXYL
We dissolved CxP (1.00 g, 5.37 mmol) and PyBOP (2.79 g, 5.37 mmol) in dry tetrahydrofuran (50 mL) containing triethylamine (0.5 mL). The mixture was stirred for 30 min at room temperature. Ethylenediamine (2 mL, 74.9 mmol) was added and the mixture stirred for a further 24 h in a nitrogen atmosphere. The resulting mixture was purified using silica gel column chromatography (chloroform/methanol/triethylamine = 90/10/1). The compound of interest was identified by electrospray ionization mass spectrometry: m/z: calcd for C11H23N3O2 (M + H+), 229.2; found, 229.2. The compound was obtained as a pale-yellow liquid (yield: 37.7%).
Animals and dietary treatments
Animal protocols and dietary treatments were as described in our previous report17. All animal experiments and protocols were approved by the Ethics Committee for Animal Experiments of Kyushu University and were conducted in accordance with the Guidelines of the Committee for Care and Use of Laboratory Animals, Kyushu University. Male C57BL/6 mice 5 weeks of age were purchased from Charles River Laboratories Japan, Inc. (Yokohama, Japan), and housed in temperature and light-controlled chambers (24 °C, 12 h/12 h light/dark cycle). Before starting experiments, all animals were acclimatised for 1 week and allowed water and appropriate food (MF diet, Oriental Yeast Co., Tokyo, Japan) ad libitum. At the age of 6 weeks, they were divided into five groups (n = 5), three groups received a normal diet and two were fed a methionine-choline-deficient (MCD) diet (Oriental Yeast Co., Tokyo, Japan) for 5 weeks to create a non-alcoholic steatohepatitis (NASH) model17. Mice were fasted overnight prior to any measurements.
Evaluation of redox reaction of the probe with antioxidants
We used L-ascorbic acid to evaluate the redox reaction of the probe with antioxidants. We prepared 2.0 mM of each probe (CmP, CxP, or EDA-PROXYL) in phosphate-buffered saline (PBS). Next, a 20 mM solution of L-ascorbic acid sodium salt was prepared in PBS and both solutions were mixed at a ratio of 1:1, so that the final concentration of probe 1 mM and ascorbic was 10 mM. The time of mixing was taken as 0 min, and the electron spin resonance (ESR) spectrum was recorded every 1.5 min from 1.5 min to 15 min. Measurement parameters of X-band EPR were as follows: microwave frequency, 1 GHz; microwave power, 1 mW; the centre of the field, 336.2 mT; modulation width, 0.3 × 0.1 mT; sweep time, 60 s; sweep width, 5.0 mT; and time constant, 0.03 s.
Assessment of probe metabolism in liver tissue using X-band electron paramagnetic resonance
For EPR, we used a similar method as described in our previous study17. In brief, liver tissue was excised from C57BL/6 N mice and the surface of the tissue washed with homogenate buffer containing 70 mM sucrose, 220 mM mannitol, 2 mM HEPES, 1 mM EGTA, 0.2% bovine serum albumin, 10 mM MgCl2 and 20 mM KH2PO4, pH 7.2. Then, homogenate buffer was added to liver samples at a ratio of 4:1 (w/w) buffer: liver followed by homogenization at 1,000 rpm. The resulting homogenate was centrifuged at 8500×g and 4 °C for 10 min to remove cell membranes. The supernatant was collected and centrifuged again at 10,000×g and 4 °C for 7 min to remove the mitochondria. The supernatant was recovered and used as the cytosol fraction. The reduction rate of probe radicals was measured by X-band EPR at 5 min after mixing of probes (50 μM) and liver homogenates. Furthermore, after the addition of KCN into homogenate solution and cytosol fraction, the radical concentrations were detected by X-band EPR. Measurement parameters for X-band EPR were as follows: microwave frequency, 1 GHz; microwave power, 1 mW; centre of the field, 336.3 mT; modulation width, 0.3 × 0.1 mT; sweep time, 60 s; sweep width, 5.0 mT; and time constant, 0.03 s.
Quantitative analysis of oxidized and total probe levels in liver tissue
We dissolved CmP, CxP, and EDA-PROXYL separately in half saline to a final probe concentration of 150 mM. Overnight fasted C57BL/6 N mice received CmP, CxP, or EDA-PROXYL intravenously at a dose of 5 μL/g of body weight. After 5 min, the mice were sacrificed and the livers excised. Twice the liver volumes of half saline were added to each liver and livers were homogenized at 2,000 rpm. The concentration of radicals in the samples was measured using X-band EPR and the total concentration of CmP, CxP, or EDA-PROXYL was measured after the addition of ferricyanide. Measurement parameters of X-band EPR were as follows: microwave frequency, 1 GHz; microwave power, 1 mW; the centre of the field, 336.3 mT; modulation width, 0.3 × 0.1 mT; sweep time, 60 s; sweep width, 5.0 mT; and time constant, 0.03 s.
Redox metabolic imaging using in-vivo dynamic nuclear polarization-magnetic resonance imaging
Redox metabolic imaging was carried out using a low magnetic field for in vivo DNP-MRI as described in our previous report17. In brief, a rectangular, one-turned, curved-surface coil (longitudinal length, 20 mm; lateral length, 32 mm) was used for EPR illumination. During the procedure, the body temperature of the mice was kept at 37 ± 1 °C with a heating pad. Mice were anesthetized with 2% isoflurane and then fixed in a dorsal recumbent position on a customized holder using adhesive skin tape. The holder was placed in centre of the resonator and in vivo DNP-MRI scanning of the upper abdomen was performed immediately after intravenous injection of CmP, CxP, or EDA-PROXYL. Pharmacokinetic DNP-MRI images of mice were taken at 1-min intervals until 10 min after administration. Normal MRI images with 1 and 10 accumulations were taken without EPR irradiation. The in vivo redox map was obtained from the slope of the enhanced DNP image intensity of each pixel from four pharmacokinetic images using a custom Excel macro program. Scanning conditions for in vivo DNP-MRI were as follows: power of EPR irradiation, 7 W; flip angle, 90°; repetition time (TR) × echo time (TE) × EPR irradiation time (TEPR), 500 × 25 × 250 ms; number of accumulations, 2; slice thickness, 100 mm including the whole thickness of the mouse; phase-encoding steps, 32; field of view (FOV), 40 × 40 mm; and matrix size, 64 × 64 after reconstruction. The in vivo DNP-MRI data of both normal and NASH-model mice were analysed using Image J software.
Results and discussion
The radical compounds, CmP and CxP are reported to show as contrast agent for DNP-MRI or EPR in diagnosis of many diseases, based on its functionality tissue redox activity28. Although, these compounds have contrast activity, but the cell membrane permeability and tissue redox activity of CxP are lower compared to CmP27. CmP has moderate reactivity and very low toxicity in living tissue32. Therefore, to obtain 3D fast imaging of redox metabolism for the diagnosis of liver diseases including steatohepatitis, considerable accumulation of nitroxyl probe in the liver is required. We aimed to synthesize a novel probe using the basic skeleton of CmP to increase the rate of liver accumulation and thus enhance the radical signal. After intravenous administration, nitroxyl probes are distributed throughout different organs, but rapid uptake of the probe with liver parenchymal cells is required to achieve sufficient distribution in liver tissue for imaging. Therefore, two properties are considered for the selection of our probe. The increased selectivity for hepatic cells and enhanced accumulation property in liver tissue, targeting the transporters on the cell membrane. Various transporters exist in hepatocyte cell membranes which efficiently translocate functional molecules from the blood into hepatocytes33. We developed the EDA-PROXYL probe (Fig. 1) with the expectation that uptake would occur via the organic cation transporter-1 (OCT 1) system which plays an important role in transporting bioactive molecules across the inner membrane of mitochondria34. In addition, we investigated the sensitivity of our novel probe for liver tissue with the goal of enabling detection of the redox state of the mitochondria.
Ascorbic acid acts as a reducing agent in various enzymatic and non-enzymatic reactions35 as well as playing a significant role in the reduction of nitroxyl radical in hepatocytes36. Many nitroxyl probes react with ascorbic acid losing their free radical through reduction. Our evaluation of the redox reduction of nitroxyl radicals of CmP, CxP and EDA-PROXYL with ascorbic acid using X-band EPR revealed the redox reaction between CxP and ascorbic acid to be slower than either CmP and EDA-PROXYL with ascorbic acid. In contrast, the redox reactions of CmP and EDA-PROXYL were similar between 1 to 15 min, but the rate of reduction of EDA-PROXYL was slower than that of CmP (Fig. 2a, b).
We evaluated redox metabolism by analysing the redox reaction between the probes and liver homogenates using X-band EPR. The EPR signal of all the probes was reduced in the freshly prepared liver homogenates at 5 min, and the EPR signals of CmP and CxP were significantly lower than that of EDA-PROXYL (Fig. 3a, b). The addition of KCN, an inhibitor of complex IV in the mitochondrial electron transfer chain (ETC)37, suppressed radical metabolism in all groups of normal mice but more in mice who received EDA-PROXYL, may result from combined effect of KCN and basicity of amine (NH3+) group present in EDA-PROXYL. Furthermore, CmP, CxP, and EDA-PROXYL were not reduced by the cytosol solution (which did not contain mitochondria) (Fig. 3a, b), indicating that the redox reaction of these probes does not occur in the cytosol. These results confirm that mitochondrial redox metabolism, especially the electron transport chain in the mitochondria, is crucial for the reduction of all the probes tested. Previous reports have shown the pathology of liver disorders to be related to mitochondrial abnormalities38,39. Direct monitoring of EDA-PROXYL in the mitochondria might be a reliable method for evaluating mitochondrial function and the maintenance of this function in redox status.
The accumulation of EDA-PROXYL in the liver after intravenous administration is critical for the diagnosis of liver diseases, as sufficient DNP signal is required for adequate imaging. We assessed the extent of liver accumulation of our novel probe compared with that of CmP and CxP. Figure 4 shows the liver accumulation of the probe after intravenous administration in mice. The total concentration of each nitroxyl probe (reduced + oxidized) was calculated by analysing the oxidation of reduced probe by adding potassium ferricyanide. This showed that the accumulation of CxP40 in the liver was significantly reduced compared with the membrane-permeable probe CmP41 and EDA-PROXYL showed significantly higher accumulation in the liver compared with both CmP and CxP (by about 3- and fivefold, respectively). This suggests that the transport of EDA-PROXYL from blood into liver cells is more efficient than that of CmP and CxP. We also found that the accumulation of EDA-PROXYL in NASH-model mice was significantly different to that of CmP (Supplementary Fig. S1). Therefore, increased liver accumulation of EDA-PROXYL is universal, possibly via the cation transporter system in the mitochondrial membranes of hepatocytes.
Our in vivo DNP MRI study of the upper abdomen of living mice after intravenous administration of the probes revealed clear DNP enhancement in this region immediately after intravenous administration for all probes. Furthermore, the distributions and metabolic rates in the liver were different for each probe (Fig. 5a, b). The DNP enhancement of EDA-PROXYL showed the distribution throughout the liver region; the decay of image intensity was faster than that of the other two probes although the initial image intensity in the liver region was not different between the probes. The distribution and metabolic rates of CmP and EDA-PROXYL were also checked in NASH model and the result showed a higher distributions and metabolic rates for EDA-PROXYL in the liver tissue compared to CmP (Supplementary Fig. S2). Various types of transporters such as organic anion transporter (OATP), OCT 1, and OCTN 142,43 exist on the basolateral membrane of hepatocytes of both mice and humans. The carnitine/organic cation transporter OCTN1, classified as a xenobiotic transporter, is ubiquitously expressed in the body44,45 and transports various therapeutic agents including organic cations and zwitterions44,46,47. This transporter is functionally expressed in mouse liver tissue48,49,50 and we speculated that EDA-PROXYL was mainly distributed in the liver region due to the presence of the carnitine-based transporter system. In contrast, CxP did not accumulate significantly in the liver due to its lower membrane permeability (Fig. 5a, b)28 and rapid reduction within the circulating blood caused by the interaction with several redox factors released from tissues27.
Conclusions
We have developed and evaluated a novel molecular probe, EDA-PROXYL, which can be used to detect mitochondrial redox status in the context of liver disease. The membrane permeability of EDA-PROXYL improved in aqueous environments, reflected by the increased accumulation in liver tissue compared with CmP. The increased sensitivity and enhanced accumulation of EDA-PROXYL highlights its potentiality for determination of the redox status of mitochondria. Thus, this novel probe could be a useful and potential therapeutic approach for precise detection of redox metabolism in the mitochondria for the diagnosis of liver disease.
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Acknowledgements
This work was supported by the Medical Research and Development Programs Focused on Technology Transfer, Development of Advanced Measurement and Analysis Systems (SENTAN) from the Japan Agency for Medical Research and Development, AMED Grant Number 162128; Health Labour Sciences Research Grant (Research on Publicly Essential Drugs and Medical Devices) from the Ministry of Health, Labour and Welfare of Japan; and Special Coordination Funds for Promoting Science and Technology (SCF funding program “Innovation Center for Medical Redox Navigation”). This work was also supported by JSPS KAKENHI (Grant Numbers 16H05079 and 19H03358). We thank Amy Phillips, PhD, from Edanz Group (https://en-author-services.edanzgroup.com/ac) for editing a draft of this manuscript.
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Hosain, M.Z., Hyodo, F., Mori, T. et al. Development of a novel molecular probe for the detection of liver mitochondrial redox metabolism. Sci Rep 10, 16489 (2020). https://doi.org/10.1038/s41598-020-73336-1
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DOI: https://doi.org/10.1038/s41598-020-73336-1
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