Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

MICROSCOPY

Single-molecule FRET on its way to structural biology in live cells

Nature Methods volume 18, pages 344–345 (2021)Cite this article

Subjects

Single-molecule FRET is making stepwise progress toward the realization of its full potential: becoming the reference technique to monitor protein structural dynamics in live cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Toward distance and dynamic measurements in living cells using smFRET.

References

  1. Lerner, E. et al. Science 359, eaan1133 (2018).

    Article  Google Scholar 

  2. Asher, W. B. et al. Nat. Methods https://doi.org/10.1038/s41592-021-01081-y (2021).

  3. Sustarsic, M. & Kapanidis, A. N. Curr. Opin. Struct. Biol. 34, 52–59 (2015).

    Article  CAS  Google Scholar 

  4. Ha, T. & Tinnefeld, P. Annu. Rev. Phys. Chem. 63, 595–617 (2012).

    Article  CAS  Google Scholar 

  5. Isselstein, M. et al. J. Phys. Chem. Lett. 11, 4462–4480 (2020).

    Article  CAS  Google Scholar 

  6. Hellenkamp, B. et al. Nat. Methods 15, 669–676 (2018).

    Article  CAS  Google Scholar 

  7. Nikić, I. & Lemke, E. A. Curr. Opin. Chem. Biol. 28, 164–173 (2015).

    Article  Google Scholar 

  8. Kozma, E. & Kele, P. Org. Biomol. Chem. 17, 215–233 (2019).

    Article  CAS  Google Scholar 

  9. Kim, N. et al. Chem. Commun. (Camb.) 54, 9667–9670 (2018).

    Article  CAS  Google Scholar 

  10. Lerner, E. et al. FRET-based dynamic structural biology: challenges, perspectives and an appeal for open-science practices. eLife https://doi.org/10.7554/eLife.60416 (2021).

Download references

Author information

Authors and Affiliations

  1. Centre de Biologie Structurale (CBS), CNRS, INSERM, University Montpellier, Montpellier, France

    Robert B. Quast & Emmanuel Margeat

Authors
  1. Robert B. Quast
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emmanuel Margeat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Emmanuel Margeat.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Quast, R.B., Margeat, E. Single-molecule FRET on its way to structural biology in live cells. Nat Methods 18, 344–345 (2021). https://doi.org/10.1038/s41592-021-01084-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-021-01084-9

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing