Abstract
The translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNAs to ribosomes by recognizing the tRNA acceptor and T stems. However, the unusual truncation observed in some animal mitochondrial tRNAs seems to prevent recognition by a canonical EF-Tu. For instance, nematode mitochondria contain tRNAs lacking a T or D arm. We recently found an atypical EF-Tu (EF-Tu1) specific for nematode mitochondrial tRNAs that lack the T arm. We have now discovered a second factor, EF-Tu2, which binds only to tRNAs that lack a D arm. EF-Tu2 seems unique in its amino acid specificity because it recognizes the aminoacyl moiety of seryl-tRNAs and the tRNA structure itself. Such EF-Tu evolution might explain tRNA structural divergence in animal mitochondria.
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References
Forchhammer, K., Leinfelder, W. & Böck A. Nature 342, 453–456 (1989).
Nissen, P. et al. Science 270, 1464–1472 (1995).
Nissen, P., Thirup, S., Kjeldgaard, M. & Nyborg, J. Structure 7, 143–156 (1999).
Okimoto, R., Macfarlane, J.L., Clary, D.O. & Wolstenholme, D.R. Genetics 130, 471–498 (1992).
Watanabe, Y. et al. J. Biol. Chem. 269, 22902–22906 (1994).
Keddie, E.M., Higazi, T. & Unnasch, T.R. Mol. Biochem. Parasitol. 95, 111–127 (1998).
Ohtsuki, T. et al. J. Biol. Chem. 276, 21571–21577 (2001).
The C. elegans Sequencing Consortium. Science 282, 2012–2018 (1998).
Krause, M. & Hirsh, D. Cell 49, 753–761 (1987).
Hartl, F.U., Pfanner, N., Nicholson, D.W. & Neupert, W. Biochim. Biophys. Acta 988, 1–45 (1989).
Andersen, G.R., Thirup, S., Spremulli, L.L. & Nyborg, J. J. Mol. Biol. 297, 421–436 (2000).
Kjeldgaard, M., Nissen, P., Thirup, S. & Nyborg, J. Structure 1, 35–50 (1993).
Berchtold, H. et al. Nature 365, 126–132 (1993).
Rudinger, J., Blechschmidt, B., Ribeiro, S. & Sprinzl, M. Biochemistry 33, 5682–5688 (1994).
LaRiviere, F.J., Wolfson, A.D. & Uhlenbeck, O.C. Science 294, 165–168 (2001)
Lathe, W.C. III & Bork, P. FEBS Lett. 502, 113–116 (2001)
Miller, D.L., Nagarkatti, S., Laursen, R.A., Parker, J. & Friesen, J.D. Mol. Gen. Genet. 159, 57–62 (1978).
Olsthoorn-Tieleman, L.N., Plooster, L.J. & Kraal, B. Eur. J. Biochem. 268, 3807–3815 (2001)
Maeda, I., Kohara, Y., Yamamoto, M. & Sugimoto, A. Curr. Biol. 11, 171–176 (2001)
Fraser, A.G. et al. Nature 408, 325–330 (2000).
Sprinzl, M., Horn, C., Brown, M., Ioudovitch, A. & Steinberg, S. Nucleic Acids Res. 26, 148–153 (1998).
Yamazaki, N. et al. Genetics 145, 749–758 (1997).
Masta, S.E. Mol. Biol. Evol. 17, 1091–1100 (2000).
Woriax, V.L., Burkhart, W. & Spremulli, L.L. Biochim. Biophys. Acta 1264, 347–356 (1995).
Schwartzbach, C.J. & Spremulli, L.L. J. Biol. Chem. 264, 19125–19131 (1989).
Williams, R.J., Nagel, W., Roe, B. & Dudock, B. Biochem. Biophys. Res. Commun. 60, 1215–1221 (1974).
Janiak, F. et al. Biochemistry 29, 4268–4277 (1990).
Lodder, M., Golovine, S. & Hecht, S. J. Org. Chem. 62, 778–779 (1997).
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. Nucleic Acids Res. 24, 4876–4882 (1997).
Acknowledgements
We thank A. Coulson (Sanger Centre, UK) for the cDNA clone, K. Kita (University of Tokyo) for A. suum and T. Hanada for E. coli AlaRS. Supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan (to K.W.) and Grant-in-Aid for Encouragement of Young Scientists (to T.O.).
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Ohtsuki, T., Sato, A., Watanabe, Yi. et al. A unique serine-specific elongation factor Tu found in nematode mitochondria. Nat Struct Mol Biol 9, 669–673 (2002). https://doi.org/10.1038/nsb826
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DOI: https://doi.org/10.1038/nsb826
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