Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Bigger is better: megadalton protein NMR in solution

Nature Structural Biology volume 9, pages 567–568 (2002)Cite this article

NMR spectra recorded on the 900 kDa GroEL–GroES complex substantially raise the bar for the size of macromolecules that can be studied by NMR techniques.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Fiaux, J., Bertelsen, E.B., Horwich, A.L. & Wüthrich, K. Nature 418, 207–211 (2002).

    Article  CAS  Google Scholar 

  2. Gardner, K.H. & Kay, L.E. Annu. Rev. Biophys. Biomol. Struct. 27, 357–406 (1998).

    Article  CAS  Google Scholar 

  3. Pervushin, K., Riek, R., Wider, G. & Wüthrich, K. Proc. Natl. Acad. Sci. USA 94, 12366–12371 (1997).

    Article  CAS  Google Scholar 

  4. Salzmann, M., Pervushin, K., Wider, G., Senn, H. & Wüthrich, K. J. Am. Chem. Soc. 122, 7543–7548 (2000).

    Article  CAS  Google Scholar 

  5. Morris, G.A. & Freeman, R. J. Am. Chem. Soc. 101, 760–762 (1979).

    Article  CAS  Google Scholar 

  6. Dalvit, C. J. Magn. Res. 97, 645–650 (1992).

    CAS  Google Scholar 

  7. Riek, R., Wider, G., Pervushin, K. & Wüthrich, K. Proc. Natl. Acad. Sci. USA 96, 4918–4923 (1999).

    Article  CAS  Google Scholar 

  8. Riek, R., Pervushin, K. & Wüthrich, K. Trends Biochem. Sci. 25, 462–468 (2000).

    Article  CAS  Google Scholar 

  9. Landry, S.J., Zeilstra-Ryalls, J., Fayet, O., Georgopoulos, C. & Gierasch, L.M. Nature 364, 255–258 (1993).

    Article  CAS  Google Scholar 

  10. Shewmaker, F., Maskos, K., Simmerling, C. & Landry, S.J. J. Biol. Chem. 276, 31257–31264 (2001).

    Article  CAS  Google Scholar 

  11. Xu, Z., Horwich, A.L. & Sigler, P.B. Nature 388, 741–750 (1997).

    Article  CAS  Google Scholar 

  12. Tugarinov, V., Muhandirum, R., Ayed, A. & Kay, L.E. J. Am. Chem. Soc. In the press (2002).

Download references

Acknowledgements

The authors thank E.B. Bertelsen and L.E. Kay for helpful comments and discussion.

Author information

Authors and Affiliations

  1. the Department of Chemistry and Institute of Molecular Biology, University of Oregon, Eugene, 97403, Oregon, USA

    Ian J. Griswold & Frederick W. Dahlquist

Authors
  1. Ian J. Griswold
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frederick W. Dahlquist
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Griswold, I., Dahlquist, F. Bigger is better: megadalton protein NMR in solution. Nat Struct Mol Biol 9, 567–568 (2002). https://doi.org/10.1038/nsb0802-567

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb0802-567

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing