Abstract
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
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Work related to this method has been continuously supported by the Swiss National Fonds.
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Biber, J., Stieger, B., Stange, G. et al. Isolation of renal proximal tubular brush-border membranes. Nat Protoc 2, 1356–1359 (2007). https://doi.org/10.1038/nprot.2007.156
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DOI: https://doi.org/10.1038/nprot.2007.156
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