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Single-cell genomics

Nature Methods volume 8, pages 311–314 (2011)Cite this article

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Abstract

Methods for genomic analysis at single-cell resolution enable new understanding of complex biological phenomena. Single-cell techniques, ranging from flow cytometry and microfluidics to PCR and sequencing, are used to understand the cellular composition of complex tissues, find new microbial species and perform genome-wide haplotyping.

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Figure 1: High-throughput single-cell gene expression using microfluidic chips for studying the cellular hierarchy of solid tissues and tumors.
Figure 2: Single-cell genome sequencing using microfluidics.

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Acknowledgements

We thank P. Dalerba, M. Rothenberg and M. Clarke for providing the tissue section and FACS plot images for Figure 1, and P. Blainey for preparing Figure 2 and for reading the manuscript.

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Authors and Affiliations

  1. Tomer Kalisky is in the Department of Bioengineering, Stanford University, Stanford, California, USA.,

    Tomer Kalisky

  2. Stephen R. Quake is in the Departments of Applied Physics and Bioengineering, Stanford University, Stanford, California, USA and Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.,

    Stephen R Quake

Authors
  1. Tomer Kalisky
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Correspondence to Stephen R Quake.

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Competing interests

S.R.Q. is a founder, consultant and shareholder of Fluidigm corp.

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Kalisky, T., Quake, S. Single-cell genomics. Nat Methods 8, 311–314 (2011). https://doi.org/10.1038/nmeth0411-311

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