Abstract
We mapped regulatory loci for nearly all protein-coding genes in mammals using comparative genomic hybridization and expression array measurements from a panel of mouse–hamster radiation hybrid cell lines. The large number of breaks in the mouse chromosomes and the dense genotyping of the panel allowed extremely sharp mapping of loci. As the regulatory loci result from extra gene dosage, we call them copy number expression quantitative trait loci, or ceQTLs. The −2log10P support interval for the ceQTLs was <150 kb, containing an average of <2–3 genes. We identified 29,769 trans ceQTLs with −log10P > 4, including 13 hotspots each regulating >100 genes in trans. Further, this work identifies 2,761 trans ceQTLs harboring no known genes, and provides evidence for a mode of gene expression autoregulation specific to the X chromosome.
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28 April 2008
In the version of this article initially published, the scale was inadvertently omitted from the bottom left portion of Figure 4. This error has been corrected in the PDF version of the article.
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C.C.P., A.S., A.H.K. and C.J.F. carried out the experiments; S.A., J.S.B., A.L., R.T.W. and T.W. did the statistical and computational analysis; C.C.P., A.J.L., R.M.L., K.L. and D.J.S. wrote the paper; D.J.S. devised the study.
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Park, C., Ahn, S., Bloom, J. et al. Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids. Nat Genet 40, 421–429 (2008). https://doi.org/10.1038/ng.113
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DOI: https://doi.org/10.1038/ng.113
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