Sir,
Trabeculectomy with adjunctive antifibrotic agents produces thin, avascular blebs and a greater risk of bleb leakage and infection.1, 2 These blebs usually demonstrate epithelial breakdown with goblet cell depletion.1 Decreased inflammatory response and abnormal blood flow are risk factors for blebitis and of recurrent infection.2, 3 The Rostock Cornea Module (RCM) has been successfully used to image microstructural changes in filtering blebs.4, 5 We report the RCM findings of blebitis before and 3 months after treatment.
Case report
Three years following successful trabeculectomy with mitomycin C (0.4 mg/ml for 3 min), a 78-year-old man presented with complaints of tearing, pain, and redness of 2 days duration. The bleb was avascular, thin, and partially loculated with surrounding hyperaemia. No bleb leakage was noted, but cells were present in the anterior chamber. Intraocular pressure was 12 mm Hg. Before initiating treatment, multiple transverse images of the bleb were taken using a new non-contact prototype objective lens for the RCM ( × 50 Nikon lens, 1–2 μm transverse resolution, field of view: 500 × 500 μm). Unlike quiet thick-walled blebs (Figure 1), the images of the thin inflamed bleb (Figure 2a) revealed markedly reduced epithelial and goblet cells, scattered presumed inflammatory cells, and increased stromal hyperreflectivity (Figure 2b and c). A few stromal cystic spaces were seen (Figure 2d). He was treated with fortified antibiotics and prednisolone drops, which were tapered over 3 months, after which the intraocular pressure was unchanged. No signs of bleb inflammation or leak were present (Figure 2e). Repeat confocal microscopy of the bleb just few days before discontinuing the steroids revealed increased number/size of stromal cystic spaces and the absence of the presumed inflammatory cells (Figure 2f). When compared with the images taken before treatment, the epithelial/goblet cells did not change and the stromal hyperreflectivity faded after the oedema resolved.
Comment
The non-contact RCM allows high-resolution imaging of thin or inflamed blebs without the risk of trauma. In our patient, the confocal images showed reduced epithelial cells and increased cellular infiltration, which are similar to histological findings seen in blebitis.3 It seems the inflammatory response did not adversely affect bleb filtration 3 months later, where the intraocular pressure was controlled and the aqueous-filled cystic spaces were still present and the steroid was then discontinued.
In conclusion, RCM is effective in identifying microscopic changes associated with bleb inflammation, such as cellular infiltration. It may aid clinicians in evaluating blebitis under long-term steroid use and thus bleb failure in subclinical stages.
References
Poulsen EJ, Allingham RR . Characteristics and risk factors of infections after glaucoma filtering surgery. J Glaucoma 2000; 9: 438–443.
Soltau JB, Rothman RF, Budenz DL, Greenfield DS, Feuer W, Liebmann JM et al. Risk factors for glaucoma filtering bleb infections. Arch Ophthalmol 2000; 118: 338–342.
Matsuo H, Tomita G, Araie M, Suzuki Y, Kaji Y, Obata H et al. Histopathological findings in filtering blebs with recurrent blebitis. Br J Ophthalmol 2002; 86: 827.
Messmer EM, Zapp DM, Mackert MJ, Thiel M, Kampik A . In vivo confocal microscopy of filtering blebs after trabeculectomy. Arch Ophthalmol 2006; 124: 1095–1103.
Labbé A, Dupas B, Hamard P, Baudouin C . In vivo confocal microscopy study of blebs after filtering surgery. Ophthalmology 2005; 112: 1979.
Acknowledgements
This study was supported in part by the Joseph Alexander Foundation.
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Heidelberg Engineering GmBH, Dossenheim, Germany (instrument support).
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Sbeity, Z., Radcliffe, N., Palmiero, PM. et al. Non-contact in vivo scanning laser microscopy of blebitis. Eye 23, 1615–1616 (2009). https://doi.org/10.1038/eye.2008.246
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DOI: https://doi.org/10.1038/eye.2008.246