Abstract
Adherence of bacteria to the surface of contact lenses may play an important role in contact lens intolerance and corneal infections. To decrease the capability of bacteria to adhere to contact lenses we incubated two types of soft contact lenses with two strains of Pseudomonas aeruginosa (serotypes 0:11 and 0:8) at a concentration of 5×107 c.f.u./ml for 12 hours. When heparin was added to the medium at a concentration of 1000 IU/ml the numbers of bacteria adhering to the contact lenses were significantly fewer than in the controls (p< 0.005). Our results suggest that heparin, either included in contact lens solutions or bonded to the surface of the contact lens, may decrease contact-lens-related morbidity.
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Galentine PG, Cohen EJ, Laibson PR, et al. Corneal ulcers associated with contact lens wear. Arch Ophthalmol 1984;102:891–4.
Alfonso E, Mandelbaum S, Fox MJ, et al. Ulcerative keratitis associated with contact lens wear. Am J Ophthalmol 1986;101:429–33.
Ormerod LD, Smith RE . Contact lens-associated microbial keratitis. Arch Ophthalmol 1986;104:79–82.
Chalupa E, Swarbrick HA, Holden BA, et al. Severe corneal infections associated with contact lens wear. Ophthalmology 1987;94:17–22.
Schein OD, Glynn RJ, Poggio EC, et al The relative risk of ulcerative keratitis among users of daily-wear and extended-wear soft contact lenses. N Engl J Med 1989;321:773–8.
Poggio EC, Glynn RJ, Schein OD, et al. The incidence of ulcerative keratitis among users of daily-wear and extended-wear soft contact lenses. N Engl J Med 1989;321:779–83.
Dart JK, Badenoch PR . Bacterial adherence to contact lenses. CLAO J 1986;12:220–4.
Durán JA, Refojo MF, Gipson IK, et al. Pseudomonas attachment to new hydrogel contact lenses. Arch Ophthal-mol 1987;105:106–9.
Aswad MI, John T, Barza M, et al. Bacterial adherence to extended-wear soft contact lenses. Ophthalmology 1990;97:296–302.
Holland S, Ruseka I, Alfonso E, et al. Biofilm, Pseudomonas and extended wear contact lens. Invest Ophthalmol Vis Sci 1988; (ARVO Suppl) 29:249.
Wilson LA, Schlitzer RL, Ahearn DG . Pseudomonas corneal ulcers associated with soft contact lenses wear. Am J Ophthalmol 1981;92:546–54.
Mayo MS, Cook WL, Schlitzer RL, et al Antibiograms, serotypes and plasmid profiles of P seudomonas aeruginosa associated with corneal ulcers and contact lens wear. J Clin Microbiol 1986;24:372–6.
Stern GA, Zam ZS . The pathogenesis of contact lens-associated Pseudomonas aeruginosa corneal ulceration. I. The effect of contact lens coatings on adherence of Pseudomonas aeruginosa to soft contact lenses. Cornea 1986;5:41–5.
Butrus SI, Klotz SA, Misra RP . The adherence of Pseudomonas aeruginosa to soft contact lenses. Ophthalmology 1987;94:1310–4.
Miller MJ, Wilson LA, Ahearn DG . Effects of protein, mucin and human tears on adherence of P seudomonas aeruginosa to hydrophilic contact lenses. J Clin Microbiol 1988;26:513–7.
Klotz SA, Butrus SI, Misra RP, et al The contribution of bacterial surface hydrophobicity to the process of adherence of Pseudomonas aeruginosa to hydrophilic contact lenses. Curr Eye Res 1989;8:195–202.
Durán JA, Refojo MF, Kenyon KR . Hydrogel contact lens-induced Pseudomonas keratitis in a rabbit model. Cornea 1987;6:258–60.
Aswad MI, Barza M, Baum J . Effect of lid closure on contact lens-associated Pseudomonas keratitis. Arch Ophthalmol 1989;107:1667–70.
Hanno PM, Parsons CL, Shrom SH, et al The protective effect of intravesical heparin in experimental bladder infection. J Surg Res 1978;25:324–9.
Parsons CL, Mulholland SG, Anwar H . Antibacterial activity of bladder surface mucin duplicated by exogenous glycosaminoglycan (heparin). Infect Inmun 1979;24:552–7.
Chin JL, Sharpe JR . The anti-adherence effect of heparin: a visual analysis. Urol Res 1983;11:173–9.
Ruggieri MR, Hanno PM, Levin RM . The effects of heparin on the adherence of five species of urinary tract pathogens to urinary bladder mucosa. Urol Res 1984;12:199–203.
Ruggieri MR, Hanno PM, Levin RM . Further characterisation of bacterial adherence to urinary bladder mucosa: comparison with adherence to anion exchange resin. J Urol 1985;134:1019–23.
Hanno PM, Fritz RW, Mulholland SG, et al Heparin, an examination of its antibacterial adsorption properties. Eur Urol 1981;7:359–62.
Fowler SA, Allansmith MR . Evaluation of soft contact lens coatings. Arch Ophthalmol 1980;98:95–9.
Liotet S, Guillaumin D, Coechet P, et al The genesis of organic deposits on soft contact lenses. CLAO J 1983;9:49–56.
Baleriola-Lucas C, Grant T, Newton-Howes J, et al Enumeration and identification of bacteria on hydrogel lenses from asymptomatic patients and those experiencing adverse responses with extended wear. Invest Ophthalmol Vis Sci 1991; (ARVO Suppl) 32:739.
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Durán, J., Malvar, A., Rodriguez-Ares, M. et al. Heparin inhibits pseudomonas adherence to soft contact lenses. Eye 7, 152–154 (1993). https://doi.org/10.1038/eye.1993.32
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DOI: https://doi.org/10.1038/eye.1993.32