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A TBP–TAF complex required for transcription of human snRNA genes by RNA polymerases II and III

Nature volume 374, pages 653–656 (1995)Cite this article

Abstract

THE TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs)1. In general, each of these TBP–TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively2–10. Here we characterize a fourth TBP–TAF complex called SNAPc11. Unlike the other TBP–TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes11–13. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein.

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA

    R. William Henry & Ryuji Kobayashi

  2. Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, New York, 11794, USA

    Cynthia L. Sadowski

  3. Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA

    Nouria Hernandez

Authors
  1. R. William Henry
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  2. Cynthia L. Sadowski
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  3. Ryuji Kobayashi
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  4. Nouria Hernandez
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Henry, R., Sadowski, C., Kobayashi, R. et al. A TBP–TAF complex required for transcription of human snRNA genes by RNA polymerases II and III. Nature 374, 653–656 (1995). https://doi.org/10.1038/374653a0

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