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The spindle cycle in budding yeast

Nature Cell Biology volume 3, pages E23–E27 (2001)Cite this article

Abstract

The mitotic spindle of the budding yeast Saccharomyces cerevisiae will probably be the first such organelle to be understood in molecular detail. Here we describe the mitotic spindle cycle of budding yeast using electron-microscope-derived structures and dynamic live-cell imaging. Recent work has revealed that many general aspects of mitosis are conserved, making budding yeast an excellent model for the study of mitosis.

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Figure 1: Schematic diagram of the yeast cell cycle.
Figure 2: Two views of the duplication plaque.
Figure 3: Transient separation of sister chromatids.

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Acknowledgements

We are indebted to C. Pearson and K. Bloom for Fig. 3 and for sharing unpublished data. We thank S. Jones for critical reading of the manuscript, and members of our laboratories for support for our structural analysis. Our structural work is supported by the National Institutes of Health (grant nos GM51312 & GM59992) and the March of Dimes Birth Defects Foundation (FY00-55). The Boulder Laboratory for 3D Fine Structure is a National Research Resource Center (RR00592) under the direction of J. R. McIntosh.

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Authors and Affiliations

  1. MCD Biology, CB 347, University of Colorado, Boulder, 80309, Colorado, USA

    Mark Winey & Eileen T. O'Toole

  2. Boulder Laboratory for 3D Fine Structure, CB 347, University of Colorado, Boulder, 80309, Colorado, USA

    Eileen T. O'Toole

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  1. Mark Winey
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Supplementary information

Movie 1a

Duplicating spindle pole body. Serial 2.3-nm tomographic slices of a duplicating SPB in a 300-nm section, as shown in Fig. 2b, animated to allow the viewer to see the series of images through the structure. (MOV 3807 kb)

Movie 1b

Duplicating spindle pole body. The same series of images with model points added. Nuclear (dark blue) and cytoplasmic (light blue) microtubules are indicated, as are the two layers of the duplication plaque (red) and the central plaque of the existing SPB (yellow). These model points are used to generate a three-dimensional model as shown in Fig 1b. (MOV 5241 kb)

Movie 2

Time-lapse movie of transient kinetochore splitting. Images were obtained at 1-s intervals of cell similar to that in Fig. 3 with LacI repressor-GFP bound to a Lac-operator cluster 1 kb from the centromere on chromosome XI; the positions of the SPBs are marked by Spc72-GFP. Selected in-focus frames were used to compose the movie, which includes elapsed time. (MOV 7891 kb)

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Winey, M., O'Toole, E. The spindle cycle in budding yeast. Nat Cell Biol 3, E23–E27 (2001). https://doi.org/10.1038/35050663

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