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Successful vaccination with a polyvalent live vector despite existing immunity to an expressed antigen

Nature volume 335, pages 259–262 (1988)Cite this article

Abstract

A global vaccination strategy must take into account production and delivery costs as well as efficacy and safety. A heat-stable, polyvalent vaccine that requires only one inoculation and induces a high level of humoral and cellular immunity against several diseases is therefore desirable. A new approach is to use live microorganisms such as mycobacteria1, enteric bacteria2,3, adenoviruses4, herpes viruses5,6 and poxviruses7 as vaccine vectors. A potential limitation of live polyvalent vaccines, however, is existing immunity within the target population not only to the vector, but to any of the expressed antigens. This could restrict replication of the vector, curtail expression of antigens, and reduce the total immune response to the vaccine. Recently acquired immunity to vaccinia virus can severely limit the efficacy of a live recombinant vaccinia-based vaccine8, so a strategy involving closely spaced inoculations with the same vector expressing different antigens may present difficulties. We have constructed a recombinant vaccinia virus that expresses surface proteins from two diverse pathogens, influenza A virus haemagglutinin and herpes simplex virus type 1 (HSV-1) glycoprotein D. Mice that had recently recovered from infection with either HSV-1 or influenza A virus could still be effectively immunized with the double recombinant.

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Author notes

  1. Vadim Yuferov

    Present address: D. I. Ivanovsky Institute of Virology, Moscow, USSR

Authors and Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Charles Flexner, Vadim Yuferov & Bernard Moss

  2. Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Brian R. Murphy

  3. Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, 20892, USA

    James F. Rooney, Charles Wohlenberg & Abner L. Notkins

Authors
  1. Charles Flexner
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  2. Brian R. Murphy
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  3. James F. Rooney
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  4. Charles Wohlenberg
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  5. Vadim Yuferov
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  6. Abner L. Notkins
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  7. Bernard Moss
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Flexner, C., Murphy, B., Rooney, J. et al. Successful vaccination with a polyvalent live vector despite existing immunity to an expressed antigen. Nature 335, 259–262 (1988). https://doi.org/10.1038/335259a0

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